FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1388493
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCACCGCCGAAGAACAC64350.4634521023872644No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT61650.4440065596297569No Hit
CTCCTGGACAGTCGATCTCCATCTCCTGCATTTGTGCTTATACCTCTGTCTCCTGGTATCAACAACACCCAGGCA46740.336623951291076No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTATGTCTCATAAGT44860.3230840918895522No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCTCCATCTCCTG37520.2702210238006241No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC37400.269356777455846No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC35410.2550246922382756No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCAC34870.2511355836867741No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA34480.2483267830662452No Hit
GATTATTACTGCAGCTCATATGCAAGCGGCAACGTGTTCTTCGGCGGTGGGACCAAGCTGACCGTCCTGGGTC32760.23593925212442554No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG30710.22117504373446606No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28660.2064108353445066No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28520.20540254794226545No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTATGTCTCATAAGTGACTTCTACCCGGG28180.20295384996539415No Hit
GGTATGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA27690.19942484405755015No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT26510.19092642166723203No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC25950.1868932720582675No Hit
CTTATGAGACATACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25900.18653316941460993No Hit
GACCCAGGACGGTCAGCTTGGTCCCACCGCCGAAGAACACGTTGCCGCTTGCATATGAGCTGCAGTAATAATC25860.1862450872996839No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25800.18581296412729484No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC25600.18437255355266466No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCTC25090.18069950658735767No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCTCCATCTCCTGCATTTGTGCTTATACCT25080.18062748605862616No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCCGAGATGGTCAGGGAGGCCGTGTTGCCGGGCTTGGAGCCAG25040.1803394039437001No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG24370.17551402851868897No Hit
ACCCAGGACGGTCAGCTTGGTCCCACCGCCGAAGAACACGTTGCCGCTTGCATATGAGCTGCAGTAATAATCAGC24220.17443372058771633No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA24030.17306533054181764No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTATGTCTCATAAGTGACTTCTACC23190.16701560612837082No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22630.1629824565194063No Hit
GCCTGGAGCCCCGAGATGGTCAGGGAGGCCGTGTTGCCGGGCTTGGAGCC22270.16038971748507194No Hit
CCCCTGAGGGCCGATGAAAGACATCAAAAATTATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGATACCAGG21360.15383584937050457No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC20990.1511710898074387No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACATACCAGTGTGGCCTTGTTGGCTTGA20850.15016280240519758No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCTCCATCTCCTGCATTTGTGCTTATAC20480.14749804284213172No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC20290.14612965279623305No Hit
GTCGATCTCCATCTCCTGCATTTGTGCTTATACCTCTGTCTCCTGGTATCAACAACACCCAGGCAAAGCCCCCAA20140.1450493448652604No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG19840.14288872900331512No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGC19840.14288872900331512No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC19830.14281670847458358No Hit
GTAATATATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGGCGGTGTCGATGGACTTGTCGGCCG19080.13741516881972038No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18920.13626284036001624No Hit
ATATATTACTGTGCGAGAGATACGAGAGTAACTTATGGTGTTTCGTATTCGGGTCCGAAATTCGGTTTGGACGT18800.1353985940152381No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCTCCATCTCCTGCATTTGTGCTT18800.1353985940152381No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18440.13280585498090375No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGAC18190.13100534176261602No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT18000.12963695171671732No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17990.12956493118798582No Hit
GGTATCAACAACACCCAGGCAAAGCCCCCAAACTCATAATTTTTGATGTCTTTCATCGGCCCTCAGGGGTTTCTG17740.1277644179696981No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT17450.1256758226364843No Hit
CTCCCTGACCATCTCGGGGCTCCAGGCTGAGGACGAGGCTGATTATTACT17440.1256038021077528No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCCCAGGACCCTGTGCCCTGAGTGAGG17250.12423541206185412No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC17180.12373126836073355No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCATAATTTTTGATGTCTTTCATCGGCCCTCAGGGGTTTCTGATCG17000.12243489884356637No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA16930.12193075514244579No Hit
GGTCAGGGAGGCCGTGTTGCCGGGCTTGGAGCCAGAGAAGCGATCAGAAACCCCTGAGGGCCGATGAAAGACATC16920.1218587346137143No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC16890.12164267302751976No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16870.12149863197005674No Hit
CATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCCGAG16730.1204903445678156No Hit
AGACATACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC16480.11868983134952786No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16370.11789760553348126No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16360.11782558500474975No Hit
GCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATGCAAGCGGCAACGTGTTCTTCGGCGGTGGGAC16320.1175375028898237No Hit
GCTTATACCTCTGTCTCCTGGTATCAACAACACCCAGGCAAAGCCCCCAAACTCATAATTTTTGATGTCTTTCAT16170.11645719495885108No Hit
CTGGTATGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTG15760.11350435328085919No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15690.11300020957973861No Hit
GCTTGGTCCCACCGCCGAAGAACACGTTGCCGCTTGCATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGC15610.11242404534988652No Hit
ACATACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGA15580.11220798376369201No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGT15090.10867897785584804No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14910.10738260833868085No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACATACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG14750.1062302798789767No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14640.10543805406293011No Hit
GCTCATATGCAAGCGGCAACGTGTTCTTCGGCGGTGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCC14600.10514997194800407No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCTCCATCTCCTGCA14440.10399764348829992No Hit
GGCTTGGAGCCAGAGAAGCGATCAGAAACCCCTGAGGGCCGATGAAAGAC14420.1038536024308369No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC14390.10363754084464236No Hit
TACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14380.10356552031591083No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCACCGCCGAAGAACACGTTGCCG14360.10342147925844782No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACATACCAGTGTGGC14250.10262925344240123No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14240.10255723291366971No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCAC14160.10198106868381764No Hit
AGGCCACACTGGTATGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14050.10118884286777104No Hit
GGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATGCAAGCGGCAACGTGTTCTTCGGCGGTGGGACCA13980.10068469916665046No Hit
GTTGATACCAGGAGACAGAGGTATAAGCACAAATGCAGGAGATGGAGATCGACTGTCCAGGAGACCCAGACACGG13980.10068469916665046No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13950.10046863758045595No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)