FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1388493
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCACCGCCGAAGAACAC60700.43716460940026347No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT60490.4356521782969017No Hit
CTCCTGGACAGTCGATCTCCATCTCCTGCATTTGTGCTTATACCTCTGTCTCCTGGTATCAACAACACCCAGGCA45430.32718926202724824No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTATGTCTCATAAGT41200.29672457837381966No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC38760.2791515693633313No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA38060.2741101323521256No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCTCCATCTCCTG37970.2734619475935421No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCAC32800.23622733423935158No Hit
GATTATTACTGCAGCTCATATGCAAGCGGCAACGTGTTCTTCGGCGGTGGGACCAAGCTGACCGTCCTGGGTC30370.21872634575759473No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC30020.21620562725199194No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28440.20482638371241338No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT28390.20446628106875583No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG28160.2028098089079311No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCTC27420.19748028978179938No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC25660.1848046767250537No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC25550.18401245090900709No Hit
GGTATGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA25330.1824279992769139No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25310.18228395821945087No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25180.18134769134594125No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTATGTCTCATAAGTGACTTCTACCCGGG24550.17681039803585616No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC23960.17256118684069707No Hit
GACCCAGGACGGTCAGCTTGGTCCCACCGCCGAAGAACACGTTGCCGCTTGCATATGAGCTGCAGTAATAATC23490.16917622199031612No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG23310.16787985247314893No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCTCCATCTCCTGCATTTGTGCTTATACCT23030.16586327766866668No Hit
CTTATGAGACATACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22870.16471094920896254No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCCGAGATGGTCAGGGAGGCCGTGTTGCCGGGCTTGGAGCCAG22020.15858920426678422No Hit
ACCCAGGACGGTCAGCTTGGTCCCACCGCCGAAGAACACGTTGCCGCTTGCATATGAGCTGCAGTAATAATCAGC21720.15642858840483892No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTATGTCTCATAAGTGACTTCTACC21560.15527625994513475No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG21430.15433999307162516No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA21020.15138715139363323No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC20610.14843430971564134No Hit
CCCCTGAGGGCCGATGAAAGACATCAAAAATTATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGATACCAGG20390.14684985808354814No Hit
GCCTGGAGCCCCGAGATGGTCAGGGAGGCCGTGTTGCCGGGCTTGGAGCC20240.14576955015257548No Hit
GTCGATCTCCATCTCCTGCATTTGTGCTTATACCTCTGTCTCCTGGTATCAACAACACCCAGGCAAAGCCCCCAA20150.1451213653939919No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCTCCATCTCCTGCATTTGTGCTTATAC19950.14368095481936172No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCTCCATCTCCTGCATTTGTGCTT19950.14368095481936172No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACATACCAGTGTGGCCTTGTTGGCTTGA19830.14281670847458358No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC19660.14159235948614793No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG19160.13799133304957245No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19090.1374871893484519No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGC18640.13424626555553393No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT18450.13287787550963526No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17920.12906078748686525No Hit
GTAATATATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGGCGGTGTCGATGGACTTGTCGGCCG17710.12754835638350356No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC17700.12747633585477205No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGAC17630.12697219215365146No Hit
GGTATCAACAACACCCAGGCAAAGCCCCCAAACTCATAATTTTTGATGTCTTTCATCGGCCCTCAGGGGTTTCTG17530.12625198686633637No Hit
ATATATTACTGTGCGAGAGATACGAGAGTAACTTATGGTGTTTCGTATTCGGGTCCGAAATTCGGTTTGGACGT17370.12509965840663223No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC17000.12243489884356637No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA16880.12157065249878826No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16800.12099448826893619No Hit
GGTCAGGGAGGCCGTGTTGCCGGGCTTGGAGCCAGAGAAGCGATCAGAAACCCCTGAGGGCCGATGAAAGACATC16700.12027428298162109No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16670.12005822139542655No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC16420.1182577081771388No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16390.11804164659094428No Hit
GCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATGCAAGCGGCAACGTGTTCTTCGGCGGTGGGAC16370.11789760553348126No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16350.11775356447601824No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCATAATTTTTGATGTCTTTCATCGGCCCTCAGGGGTTTCTGATCG16220.11681729760250861No Hit
GCTTGGTCCCACCGCCGAAGAACACGTTGCCGCTTGCATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGC16220.11681729760250861No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16010.11530486649914692No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACATACCAGTGTGGC15680.1129281890510071No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15630.11256808640734955No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCCCAGGACCCTGTGCCCTGAGTGAGG15570.11213596323496049No Hit
CTGGTATGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTG15250.10983130631555219No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGT15120.10889503944204255No Hit
GCTTATACCTCTGTCTCCTGGTATCAACAACACCCAGGCAAAGCCCCCAAACTCATAATTTTTGATGTCTTTCAT15090.10867897785584804No Hit
CTCCCTGACCATCTCGGGGCTCCAGGCTGAGGACGAGGCTGATTATTACT14920.10745462886741236No Hit
AGACATACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC14750.1062302798789767No Hit
GGCTTGGAGCCAGAGAAGCGATCAGAAACCCCTGAGGGCCGATGAAAGAC14720.10601421829278218No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCTCCATCTCCTGCA14720.10601421829278218No Hit
ACATACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGA14400.10370956137337385No Hit
GCTCATATGCAAGCGGCAACGTGTTCTTCGGCGGTGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCC14280.10284531502859574No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCACCGCCGAAGAACACGTTGCCG14230.10248521238493821No Hit
GGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATGCAAGCGGCAACGTGTTCTTCGGCGGTGGGACCA14100.10154894551142857No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14060.10126086339650253No Hit
CATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCCGAG14010.100900760752845No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)