FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1304796
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57750.4425979233535357No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT55650.4265034534134071No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39330.30142642987869367No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG38210.2928427125772918No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36120.27682488297021146No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG34270.26264642135628863No Hit
GTTTCAGACTTACCCGTGGGCGTTCGGCCAAGGGACCAAGGTGGAAGTCAAGCGAACTGTGGCTGCACCAT33840.2593508870352147No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG33540.2570516770437678No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT32340.24785483707798003No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31900.2444826624238578No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCC31550.24180025076716974No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA29650.22723858748800577No Hit
GATCTATGGTGCATCCGCTTTGTTACGTGGGGTCCCATCTAGGTTCAGCGGCAGTGGATCTGGGACAGAATTC29330.22478609683046238No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA29180.22363649183473894No Hit
GTCTGAAACTGTTGACAGTAATAAGTCGCAAAATCTTCAGGCTGCAGGCCGTTGACTGTGAGAGTGAATTCTGTC29170.2235598515016907No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGGCTCTTGCTGATACCAGGC29160.22348321116864245No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC28560.21888479118574855No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG27310.20930474955471967No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT27060.20738874122851386No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA26010.1993415062584496No Hit
GTGCAGCCACAGTTCGCTTGACTTCCACCTTGGTCCCTTGGCCGAACGCCCACGGGTAAGTCTGAAACTGTTGAC25970.19903494492625665No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCC24780.18991474529351715No Hit
ACCTAGATGGGACCCCACGTAACAAAGCGGATGCACCATAGATCAGGAGCTTAGGGGCTTTCCCTGGCTCTTG23990.18386015898270688No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23940.18347695731746572No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23690.18156094899125994No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC23290.17849533566933068No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23070.1768092483422696No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGACTTCCACCTTGGTCCCTTGGCCGAACGCCCACGGGTAAGT22820.1748932400160638No Hit
CATCTAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAGTCAACGGCCTGCAGCCTGAAGATTTTG22420.17182762669413457No Hit
GTCAACGGCCTGCAGCCTGAAGATTTTGCGACTTATTACTGTCAACAGTTTCAGACTTACCCGTGGGCGTTCGGC22280.1707546620314593No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT22080.1692218553704947No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20580.15772580541326No Hit
GAATTCACTCTCACAGTCAACGGCCTGCAGCCTGAAGATTTTGCGACTTATTACTGTCAACAGTTTCAGACTTAC20500.15711268274887416No Hit
GTATCAGCAAGAGCCAGGGAAAGCCCCTAAGCTCCTGATCTATGGTGCATCCGCTTTGTTACGTGGGGTCCCAT20470.15688276174972948No Hit
ACTTATTACTGTCAACAGTTTCAGACTTACCCGTGGGCGTTCGGCCAAGGGACCAAGGTGGAAGTCAAGCGA20150.15443027109218607No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20130.1542769904260896No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19950.15289746443122143No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC19690.1509048157719674No Hit
TGCTGATACCAGGCTAAATAATTGTTAATGGCCTGACTGGCCCGGCAAGT19580.15006177210843688No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19180.14699615878650762No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT19170.1469195184534594No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGCCATTAACAATTATTTAGCCTGGTATCAGCAAG18150.1391022044825398No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC17920.13733947682243047No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC17710.13573002982841764No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT17670.1354234684962247No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCC17660.13534682816317645No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17430.13358410050306715No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC17370.13312425850477777No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17280.13243449550734368No Hit
GTAATACACGGCTGTGTCTTCGGACCTCAGGCTGCTCAACTCCAGGTAGGCTGTACTGGTGGACATCTCCCTGGT17270.13235785517429544No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17160.1315148115107649No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC16930.12975208385065556No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16420.12584342686519578No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT16010.1227011732102183No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT15790.12101508588315722No Hit
GGACCAAGGTGGAAGTCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGA15740.12063188421791607No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15390.11794947256122795No Hit
GTAATAAGTCGCAAAATCTTCAGGCTGCAGGCCGTTGACTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG15290.11718306923074566No Hit
AGATGGGACCCCACGTAACAAAGCGGATGCACCATAGATCAGGAGCTTAGGGGCTTTCCCTGGCTCTTGCTGAT14750.11304449124614116No Hit
CCTTGGTCCCTTGGCCGAACGCCCACGGGTAAGTCTGAAACTGTTGACAGTAATAAGTCGCAAAATCTTCAGG14680.11250800891480355No Hit
GCCACAGTTCGCTTGACTTCCACCTTGGTCCCTTGGCCGAACGCCCACGG14660.11235472824870707No Hit
GTCCCATCTAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCAC14450.11074528125469421No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG14220.1089825535945849No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14180.10867599226239198No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA14090.10798622926495788No Hit
GACTTACCCGTGGGCGTTCGGCCAAGGGACCAAGGTGGAAGTCAAGCGAACTGTGGCTGCACCATCTGTCTTCAT14010.10737310660057205No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13960.10698990493533088No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC13890.10645342260399326No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC13870.1063001419378968No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13800.10576365960655919No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13740.10530381760826979No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13650.10461405461083571No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13630.10446077394473925No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13530.10369437061425693No Hit
CTCTCACAGTCAACGGCCTGCAGCCTGAAGATTTTGCGACTTATTACTGTCAACAGTTTCAGACTTACCCGTGGG13440.10300460761682285No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC13430.10292796728377464No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13430.10292796728377464No Hit
GTTACGTGGGGTCCCATCTAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAGTCAACGGCCTGC13350.10231484461938878No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13270.10170172195500292No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13270.10170172195500292No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13240.10147180095585824No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTG13220.10131852028976178No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC13060.10009227496099006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)