FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1304796
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57580.4412950376917158No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT46680.3577570746691437No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38980.2987440182220056No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG34720.26609523634345905No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA34080.26119025502837223No Hit
GTCTGAAACTGTTGACAGTAATAAGTCGCAAAATCTTCAGGCTGCAGGCCGTTGACTGTGAGAGTGAATTCTGTC33820.2591976063691182No Hit
GTTTCAGACTTACCCGTGGGCGTTCGGCCAAGGGACCAAGGTGGAAGTCAAGCGAACTGTGGCTGCACCAT32050.24563226741958127No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG31970.24501914475519543No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31640.2424900137646038No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30730.2355157434572148No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCC30490.23367637546405723No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT30340.23252677046833375No Hit
GATCTATGGTGCATCCGCTTTGTTACGTGGGGTCCCATCTAGGTTCAGCGGCAGTGGATCTGGGACAGAATTC28650.21957455418318267No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC26590.20378664557524703No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT26370.20210055824818593No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGGCTCTTGCTGATACCAGGC26310.20164071624989652No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA25960.19895830459320846No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG25040.19190739395277115No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24760.1897614646274207No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC24300.18623600930720205No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24110.18477984297928565No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCC23290.17849533566933068No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG23290.17849533566933068No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGCCATTAACAATTATTTAGCCTGGTATCAGCAAG22650.17359035435424386No Hit
CATCTAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAGTCAACGGCCTGCAGCCTGAAGATTTTG22230.17037146036621817No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21740.16661608404685482No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21470.16454679505455258No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT21390.16393367239016673No Hit
GTCAACGGCCTGCAGCCTGAAGATTTTGCGACTTATTACTGTCAACAGTTTCAGACTTACCCGTGGGCGTTCGGC21280.16309062872663618No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC21190.16240086572920212No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20870.1599483750716587No Hit
GTGCAGCCACAGTTCGCTTGACTTCCACCTTGGTCCCTTGGCCGAACGCCCACGGGTAAGTCTGAAACTGTTGAC20430.15657620041753653No Hit
GAATTCACTCTCACAGTCAACGGCCTGCAGCCTGAAGATTTTGCGACTTATTACTGTCAACAGTTTCAGACTTAC20420.1564995600844883No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC20320.155733156754006No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20150.15443027109218607No Hit
GTATCAGCAAGAGCCAGGGAAAGCCCCTAAGCTCCTGATCTATGGTGCATCCGCTTTGTTACGTGGGGTCCCAT20120.15420035009304137No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA20040.1535872274286555No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT19260.14760928145089347No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19190.14707279911955587No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGACTTCCACCTTGGTCCCTTGGCCGAACGCCCACGGGTAAGT18520.14193789680532434No Hit
TGCTGATACCAGGCTAAATAATTGTTAATGGCCTGACTGGCCCGGCAAGT18490.14170797580617966No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCC18350.14063501114350443No Hit
ACTTATTACTGTCAACAGTTTCAGACTTACCCGTGGGCGTTCGGCCAAGGGACCAAGGTGGAAGTCAAGCGA18170.13925548514863625No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC17710.13573002982841764No Hit
GTAATAAGTCGCAAAATCTTCAGGCTGCAGGCCGTTGACTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG17370.13312425850477777No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC17220.1319746535090543No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT16950.12990536451675205No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16950.12990536451675205No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC16090.12331429587460416No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16020.12277781354326654No Hit
GTCCCATCTAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCAC15590.1194822792221926No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15550.11917571788999967No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC15460.11848595489256557No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT15030.11519042057149163No Hit
GTAATACACGGCTGTGTCTTCGGACCTCAGGCTGCTCAACTCCAGGTAGGCTGTACTGGTGGACATCTCCCTGGT15030.11519042057149163No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14330.10982559725811546No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14280.10944239559287428No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14250.1092124745937296No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14060.1077563082658132No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC14020.10744974693362028No Hit
GTTACGTGGGGTCCCATCTAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAGTCAACGGCCTGC13790.10568701927351094No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13590.10415421261254633No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13480.10331116894901579No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC13410.10277468661767818No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13400.10269804628462993No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA13370.10246812528548524No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13250.10154844128890646No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC13130.1006287572923277No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG13100.100398836293183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)