FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265766
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC8560.32208785171918153No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT6850.2577455355463077No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT5980.2250099711776525No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA5100.19189813595418526No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA4670.17571848919726377No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGA4560.17157950979433034No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC4290.16142019671440289No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC4200.15803375902109373No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT4100.15427105047297246No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTG4030.15163715448928758No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT3980.14975580021522691No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC3940.14825071679597843No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGA3680.1384676745708631No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA3670.13809140371605097No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAA3620.13621004944199033No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT3570.13432869516792967No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA3500.1316947991842448No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACT3400.1279320906361235No Hit
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGC3400.1279320906361235No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCCAACCTCCGAGCAGT3350.12605073636206288No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTT3240.12191175695912945No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGC3220.1211592152495052No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA3170.11927786097544457No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC3100.11664396499175968No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC2880.10836600618589286No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTG2800.10535583934739583No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG2770.10422702678295945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)