FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851885
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT29740.3491081542696491No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29570.3471125797496141No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22220.26083332844221935No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA21580.25332057730797No Hit
GTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGACAGCCCCT21030.24686430680197444No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG20310.23841246177594397No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19750.23183880453347575No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT19460.22843458917576903No Hit
GAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGACAGCCCCTAAGCTCCTGATCTATAAGGCGTC18140.21293953996137974No Hit
GTACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACCCTGTCGCCGACAGATGCTGACAGGGTGGAAGG17540.205896335773021No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG17340.2035486010435681No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA17220.20213996020589636No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16310.19145776718688554No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC15660.18382762931616356No Hit
ACACGGGGACGTTCGGCCATGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC15410.18089296090434742No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15330.17995386701256624No Hit
TCTATAAGGCGTCCGTTTTAGGAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT14600.1713846352500631No Hit
CCTTATAGATCAGGAGCTTAGGGGCTGTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTAATACTCTG14360.16856735357471958No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA14340.16833258010177432No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG14240.16715871273704785No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACGTCC13940.16363711064286846No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA13680.16058505549457966No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACCCTGTCGCCGACAGA13680.16058505549457966No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13550.15905902792043528No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACGT13370.15694606666392766No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13240.15542003908978325No Hit
CCTTCCACCCTGTCAGCATCTGTCGGCGACAGGGTCACCATCACTTGCCG13170.15459833193447473No Hit
TCCCCGTGTTGATGCCATAAGGATTGAATTGTAGGCAGTAATAAGTTGCA13150.15436355846152944No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13150.15436355846152944No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC13080.1535418513062209No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13050.15318969109680297No Hit
ACTTATTACTGCCTACAATTCAATCCTTATGGCATCAACACGGGGACGTTCGGCCATGGGACCAAGGTGGAAATC12760.14978547573909623No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT12660.14861160837436976No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC12330.14473784607077245No Hit
TCCTAAAACGGACGCCTTATAGATCAGGAGCTTAGGGGCTGTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGT12000.14086408376717516No Hit
GCCCCTAAGCTCCTGATCTATAAGGCGTCCGTTTTAGGAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT11840.13898589598361283No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACGTCCAGATGACCCAGTCTCCTTCCACC11500.13499474694354285No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11440.13429042652470696No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11310.13276439895056258No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCATGGCCGAACGTCCCCGTGTTGATGCCATAAGGATTGA11190.13135575811289082No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA11000.12912541011991055No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC10920.1281863162281294No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT10890.12783415601871145No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10850.12736460907282085No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC10270.12055617835740738No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG10200.11973447120209887No Hit
GATCTATAAGGCGTCCGTTTTAGGAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTC10180.11949969772915359No Hit
GCTTAGGGGCTGTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTA10170.11938231099268093No Hit
GTCTCCTTCCACCCTGTCAGCATCTGTCGGCGACAGGGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAC9930.11656502931733743No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9800.11503900174319304No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC9570.11233910680432217No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9560.11222172006784953No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9500.11151739964901367No Hit
GTTTTAGGAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC9310.10928705165603339No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT9230.10834795776425221No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9200.10799579755483427No Hit
GTGTGACGTCCAGATGACCCAGTCTCCTTCCACCCTGTCAGCATCTGTCG9130.10717409039952576No Hit
GTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACC8950.10506112914301813No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCTAC8900.10447419546065491No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8840.10376987504181903No Hit
GTCAGCATCTGTCGGCGACAGGGTCACCATCACTTGCCGGGCCAGTCAGA8660.1016569137853114No Hit
GGATTGAATTGTAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC8650.10153952704883874No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)