FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851885
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34000.39911490400699623No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28020.328917635596354No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT24580.28853659824976374No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21190.24874249458553677No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA20710.24310793123484978No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT19280.22632162791926141No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA18860.22139138498741026No Hit
GTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGACAGCCCCT18360.21552204816377799No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17330.20343121430709543No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG16590.19474459580811962No Hit
GAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGACAGCCCCTAAGCTCCTGATCTATAAGGCGTC16350.19192731413277614No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16310.19145776718688554No Hit
GTACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACCCTGTCGCCGACAGATGCTGACAGGGTGGAAGG15680.18406240278910888No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC15320.1798364802760936No Hit
CCTTATAGATCAGGAGCTTAGGGGCTGTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTAATACTCTG14290.1677456464194111No Hit
TCCCCGTGTTGATGCCATAAGGATTGAATTGTAGGCAGTAATAAGTTGCA13760.16152414938636084No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG13500.15847209423807204No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13420.1575330003462909No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13250.1555374258262559No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13170.15459833193447473No Hit
ACACGGGGACGTTCGGCCATGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC13170.15459833193447473No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC13160.15448094519800207No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA13140.15424617172505678No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACCCTGTCGCCGACAGA13130.15412878498858412No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12830.15060718289440475No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG12820.15048979615793212No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12450.14614648690844423No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12340.1448552328072451No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACGT12290.14426829912488187No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC12290.14426829912488187No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACGTCC12020.14109885724012042No Hit
TCTATAAGGCGTCCGTTTTAGGAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT11710.13745986840946842No Hit
ACTTATTACTGCCTACAATTCAATCCTTATGGCATCAACACGGGGACGTTCGGCCATGGGACCAAGGTGGAAATC11490.1348773602070702No Hit
TCCTAAAACGGACGCCTTATAGATCAGGAGCTTAGGGGCTGTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGT11390.13370349284234376No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTCCTAAAACGGACGCCTTAT11160.13100359790347288No Hit
CCTTCCACCCTGTCAGCATCTGTCGGCGACAGGGTCACCATCACTTGCCG11070.12994711727521907No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG11040.12959495706580113No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC11030.1294775703293285No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA10800.12677767539045764No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC10770.1264255151810397No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA10450.12266913961391501No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACGTCCAGATGACCCAGTCTCCTTCCACC10340.12137788551271592No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10330.12126049877624327No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT10200.11973447120209887No Hit
GCCCCTAAGCTCCTGATCTATAAGGCGTCCGTTTTAGGAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT10170.11938231099268093No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10070.11820844362795448No Hit
GATCTATAAGGCGTCCGTTTTAGGAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTC9450.11093046596665043No Hit
GTCTCCTTCCACCCTGTCAGCATCTGTCGGCGACAGGGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAC9410.11046091902075984No Hit
GCTTAGGGGCTGTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTA9290.10905227818308809No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC9280.10893489144661544No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCATGGCCGAACGTCCCCGTGTTGATGCCATAAGGATTGA9000.10564806282538136No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC8880.10423942198770961No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8870.10412203525123696No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT8690.10200907399472933No Hit
GTTTTAGGAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC8670.10177430052178404No Hit
GTGTGACGTCCAGATGACCCAGTCTCCTTCCACCCTGTCAGCATCTGTCG8560.10048304642058493No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)