FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999569
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACTAAACTGTACCTGCCTGCATATGAGCAGCAGTAATAAT29630.2964277603647172No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACTAAACTGTACCTGCCTGCATATGAGC28160.2817214219328531No Hit
ATCCAGGCAAGGCCCCCAAATTCATGATTTATGATGTCAGTAAGAGGCCC27710.2772194815965681No Hit
GTACCAACACTATCCAGGCAAGGCCCCCAAATTCATGATTTATGATGTCA26100.26111253950452645No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25770.25781111659125083No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25760.2577110734726667No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24920.2493074515116015No Hit
GTGTTGGTACCAGGAGACAAAGTTATAACCACCAACATCACTGATGGTTCCAGTACAGGAGATGGTGACTG23390.23400085436823273No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGTACTGGAACCATCAGTGATGTTGGTGGTTATAACTTTGTCTCCTGGT20830.2083898160107006No Hit
CTCCTGTACTGGAACCATCAGTGATGTTGGTGGTTATAACTTTGTCTCCTGGTACCAACACTATCCAGGCAAGGC20310.2031875738443269No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA20130.20138679770981294No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC19340.19348339134166825No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGTACTGGAACCAT19200.19208278768149073No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG19020.19028201154697674No Hit
TGCCTGGATAGTGTTGGTACCAGGAGACAAAGTTATAACCACCAACATCA18130.181378173992991No Hit
GATTATTACTGCTGCTCATATGCAGGCAGGTACAGTTTAGTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGT17850.1785769666726359No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17290.17297455203192574No Hit
ATACTAAACTGTACCTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCA17240.17247433643900523No Hit
GTACTGGAACCATCAGTGATGTTGGTGGTTATAACTTTGTCTCCTGGTACCAACACTATCCAGGCAAGGCCCCCA16740.16747218050979973No Hit
GTTTAGTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC16630.1663717062053745No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16210.1621698952248419No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15960.15966881726023915No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15910.1591686016673186No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC15720.1572677824142205No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15650.15656748058413178No Hit
ACACTATCCAGGCAAGGCCCCCAAATTCATGATTTATGATGTCAGTAAGAGGCCCTCAGGGGTCCCTGATCGCTT15460.15466666133103366No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC15390.1539663595009449No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG15180.1518654540106786No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT15140.15146528153634214No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTC14930.14936437604607586No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAACAC14740.1474635567929778No Hit
GTCAGTCACCATCTCCTGTACTGGAACCATCAGTGATGTTGGTGGTTATA14190.14196118527085175No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14190.14196118527085175No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC14160.14166105591509942No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTGAGGGAGGCCGTGTTGCCAGACTTGGAGCCAG14120.14126088344076296No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14110.14116084032217885No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13840.1384596761204079No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13750.13755928805315093No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG13480.13485812385137994No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13070.13075635598943144No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGAC12920.1292557092106698No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12730.12735488995757172No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA12680.12685467436465117No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12530.1253540275858895No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC12420.1242535532814643No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG12260.12265286338411857No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACTAAACTGTACCTGCCTGCATATGAGCAGCAGTAATAATC12260.12265286338411857No Hit
CCCCTGAGGGCCTCTTACTGACATCATAAATCATGAATTTGGGGGCCTTGCCTGGATAGTGTTGGTACCAGG12210.12215264779119801No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC11990.11995169918234759No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11860.11865113864075418No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA11860.11865113864075418No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11850.11855109552217007No Hit
GGATAGTGTTGGTACCAGGAGACAAAGTTATAACCACCAACATCACTGATGGTTCCAGTACAGGAGATGGTG11820.11825096616641773No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGGTGTCCAGTCGCAGGTGC11800.11805087992924951No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11780.11785079369208129No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT11760.11765070745491306No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC11740.11745062121774484No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11540.11544975884606265No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11440.11444932766022156No Hit
GAAATACACGGCCGTGTCGTCAGATCTCAGGCTGCTCATGTCCATGTAGGCTGTGCTCGTGGATTCGTCCGCGGT11400.11404915518588511No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT11260.11264855152570757No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC11160.11164812033986647No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT11110.11114790474694594No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC11050.11054764603544127No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10990.10994738732393661No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG10980.10984734420535251No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10850.10854678366375907No Hit
CCTTGGTCCCTCCGCCGAATACTAAACTGTACCTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCCTC10830.10834669742659085No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT10690.10694609376641334No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10650.10654592129207688No Hit
GTACAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACACTGAGCGAGG10430.10434497268322646No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10420.10424492956464236No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10280.10284432590446484No Hit
AAACTGTACCTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCCTC10190.10194393783720783No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10170.10174385160003961No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10150.1015437653628714No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10050.1005433341770303No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10010.10014316170269386No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)