FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999569
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACTAAACTGTACCTGCCTGCATATGAGCAGCAGTAATAAT27030.27041654953284866No Hit
ATCCAGGCAAGGCCCCCAAATTCATGATTTATGATGTCAGTAAGAGGCCC26560.2657145229593955No Hit
GTACCAACACTATCCAGGCAAGGCCCCCAAATTCATGATTTATGATGTCA26150.26161275509744697No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACTAAACTGTACCTGCCTGCATATGAGC25970.259811978962933No Hit
GTGTTGGTACCAGGAGACAAAGTTATAACCACCAACATCACTGATGGTTCCAGTACAGGAGATGGTGACTG24210.2422043900921297No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23650.23660197545141956No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23180.2318999488779664No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22380.22389649939123765No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGTACTGGAACCATCAGTGATGTTGGTGGTTATAACTTTGTCTCCTGGT21730.2173936966832705No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA20620.2062889105204343No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG20060.20068649587972417No Hit
CTCCTGTACTGGAACCATCAGTGATGTTGGTGGTTATAACTTTGTCTCCTGGTACCAACACTATCCAGGCAAGGC19970.19978610781246717No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGTACTGGAACCAT18520.18527985561777124No Hit
TGCCTGGATAGTGTTGGTACCAGGAGACAAAGTTATAACCACCAACATCA17640.1764760611823696No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC17100.17107373277882768No Hit
ATACTAAACTGTACCTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCA17020.1702733878301548No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT16910.16917291352572958No Hit
GATTATTACTGCTGCTCATATGCAGGCAGGTACAGTTTAGTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGT16050.16056920532749613No Hit
GTACTGGAACCATCAGTGATGTTGGTGGTTATAACTTTGTCTCCTGGTACCAACACTATCCAGGCAAGGCCCCCA15960.15966881726023915No Hit
GTTTAGTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC15660.1566675237027159No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15190.15196549712926272No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC14830.14836394486023477No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14810.14816385862306652No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14650.14656316872572078No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14260.1426614871009405No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC14110.14116084032217885No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14080.14086071096642655No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTC14060.1406606247292583No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC14020.14026045225492187No Hit
ACACTATCCAGGCAAGGCCCCCAAATTCATGATTTATGATGTCAGTAAGAGGCCCTCAGGGGTCCCTGATCGCTT13940.139460107306249No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13870.13875980547616024No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAACAC13870.13875980547616024No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13430.1343579082584594No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG13330.1333574770726183No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC13030.130356183515095No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13010.1301560972779268No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTGAGGGAGGCCGTGTTGCCAGACTTGGAGCCAG13000.13005605415934268No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG12860.12865545049916516No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC12850.12855540738058105No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12710.1271548037204035No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12570.12575420006022595No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12550.12555411382305773No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12500.12505389823013718No Hit
GTCAGTCACCATCTCCTGTACTGGAACCATCAGTGATGTTGGTGGTTATA12400.12405346704429608No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12260.12265286338411857No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC12190.1219525615540298No Hit
GGATAGTGTTGGTACCAGGAGACAAAGTTATAACCACCAACATCACTGATGGTTCCAGTACAGGAGATGGTG12110.12115221660535691No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGAC11760.11765070745491306No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11710.11715049186199253No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11680.1168503625062402No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC11680.1168503625062402No Hit
CCCCTGAGGGCCTCTTACTGACATCATAAATCATGAATTTGGGGGCCTTGCCTGGATAGTGTTGGTACCAGG11650.11655023315048786No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG11490.1149495432531421No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11270.1127485946442917No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT11180.11184820657703469No Hit
GAAATACACGGCCGTGTCGTCAGATCTCAGGCTGCTCATGTCCATGTAGGCTGTGCTCGTGGATTCGTCCGCGGT11020.11024751667968896No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10960.1096472579681843No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA10900.10904699925667963No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACTAAACTGTACCTGCCTGCATATGAGCAGCAGTAATAATC10850.10854678366375907No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT10830.10834669742659085No Hit
CCTTGGTCCCTCCGCCGAATACTAAACTGTACCTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCCTC10810.10814661118942265No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA10780.10784648183367032No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG10630.10634583505490867No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGGTGTCCAGTCGCAGGTGC10610.10614574881774046No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10520.10524536075048345No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10500.10504527451331523No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10470.10474514515756292No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC10400.10404484332747414No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT10200.10204398095579195No Hit
AAACTGTACCTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCCTC10100.10104354976995084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)