FastQCFastQC Report
Wed 8 May 2024
EGAF00004717921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1537240
Total Bases112 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCGGCCCTCAGGCTGTTCATTTCCAGATAGA49980.3251281517524915No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA49480.3218755692019464No Hit
GTATATTACTGTGCGAGATATACCCCGGATGGGGAGACCTACGGTATGGA48240.3138091644765944No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACT47270.30749915432853686No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT46670.3035960552678827No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATACTCACGCAGG41640.27087507480939865No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC39260.2553927818688039No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC37700.245244724311103No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA37540.24420389789492858No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATACTCACGCAGGC37380.24316307147875413No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33610.21863859904764382No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT33330.21681715281933858No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG32470.21122271083240093No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC30260.19684629595899142No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA29780.19372381671046812No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC29670.19300824854934817No Hit
TCCAGGAACAGCCCCCAAAGTCTTCGTCTATAATAACAGGAATCGGCCCT29640.1928130935963155No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC28980.1885196846295959No Hit
GGTTATGATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAAAGT28800.18734875491139966No Hit
GATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAAAGTCTTCGT28590.18598267024017068No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA27900.1814941063204184No Hit
GTCCTATGACAACAGCCTGCGTGAGTATGTCTTCGGAACTGGGACCAAGG27830.18103874476334209No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTTCTGCCAGT26150.17011006739351045No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24550.15970180323176603No Hit
GTTCCTGGAAGGTGCTGGTACCAGTGTACATCATAACCTGCCCCGATGTT24510.15944159662772242No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG24260.15781530535244986No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC24010.15618901407717728No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23670.1539772579428066No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23630.15371705133876298No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA23310.15163539850641408No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC23230.15111498529832687No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT23160.15065962374125055No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23030.14981395227810884No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT22850.14864302255991257No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG22520.14649631807655278No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22120.1438942520361167No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGC22050.14343889047904035No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21870.1422679607608441No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG21580.14038146288152795No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC21500.1398610496734407No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA21480.1397309463714189No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCT21450.13953579141838618No Hit
GTCATAGGACTGGCAGAAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG21270.13836486170018997No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGC21200.13790950014311362No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG21190.13784444849210273No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT21150.13758424188805912No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG21110.13732403528401552No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC21060.136998777028961No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC20540.13361609117639406No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG20490.13329083292133956No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGG20080.13062371522989255No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT20050.13042856027685984No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC19630.12769639093440194No Hit
CTCCTGTGCAGCGTCTGGATTCACCTTCAGTAACTTTGTCATGCACTGGG19580.1273711326793474No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG19460.12659051286721656No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT19280.12541958314902033No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGT19270.1253545314980094No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG19250.1252244281959876No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTTCTGCCAGTCCTAT19220.1250292732429549No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC19050.12392339517576956No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG19010.12366318857172595No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18760.1220368972964534No Hit
GTGTACATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGA18610.12106112253128984No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG17940.11670266191355938No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17870.11624730035648305No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC17840.11605214540345034No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCC17780.11566183549738493No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16990.11052275506752361No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCGCCGTCTCTTCAGCATCC16870.10974213525539278No Hit
GGATGAGGCTGATTATTTCTGCCAGTCCTATGACAACAGCCTGCGTGAGT16850.10961203195337098No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC16850.10961203195337098No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTC16680.10850615388618565No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATG16430.10687986261091306No Hit
CTCCAGCCCCTTGCCTGGACCCTGGCGGACCCAGTGCATGACAAAGTTAC16380.10655460435585855No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16370.10648955270484764No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA16270.10583903619473864No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA16250.10570893289271682No Hit
GCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCACCTTCCA16230.10557882959069502No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG16140.10499336473159689No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16130.10492831308058599No Hit
GCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA16070.10453800317452057No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG16030.10427779657047695No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15980.10395253831542244No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGG15930.10362728006036792No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG15890.10336707345632432No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCAGCACTAGAAG15780.10265150529520439No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG15530.10102521401993182No Hit
GTGCTGGTACCAGTGTACATCATAACCTGCCCCGATGTTGGAGCTGCTCC15390.1001144909057792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)