FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968881
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATACTGCTGACAGTAATATGTTGCAACATCTTCAGGCTGCAGGTTGCTGATGGTGAAGGTAAAATCTGTC36320.37486543755115437No Hit
GTATTATAGTCTCCCGTACACTTTTGGCCAGGGGACCAGGCTGGAAATCAAACGAACTGTGGCTGCACCAT32290.33327106218410724No Hit
GTAATAGAGACCCGTGTCCTCAGGTATGAGGTCGTCGATTTCAAGAGATAATGTATTCTTCAAATTGTCTCTGA23740.24502493082225785No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT21920.2262403742048817No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20570.21230677451616864No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG18190.18774235432421524No Hit
CTCTATTACTGTGCGAGAGGGCGGCCTACTATGCCAGTAGAAGTGATCCT17040.17587299162642264No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16740.17277663614004196No Hit
CTATTACTGTGCGAGAGGGCGGCCTACTATGCCAGTAGAAGTGATCCTCG15860.16369399337999196No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15830.1633843578313539No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15310.1580173416549607No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGATTCAGCTTCAGTTATTACGCT15220.1570884350090465No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG14940.1541985032217579No Hit
CAGTAATAGAGACCCGTGTCCTCAGGTATGAGGTCGTCGATTTCAAGAGATAATGTATTCTTCAAATTGTCTCTG14680.1515149951335613No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13820.14263877607260333No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA13700.14140023387805106No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13630.14067775093122892No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG13440.1387167257898545No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12930.1334529214630073No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCGTAATAACTGAAGCTGAATCCAGAGGCTGCAC12660.1306662015252647No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12630.13035656597662665No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12620.1302533541270806No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT12480.12880838823343632No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCATCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTG12200.12591845644614766No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12010.12395743130477323No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC11940.12323494835795108No Hit
CAGTAATATGTTGCAACATCTTCAGGCTGCAGGTTGCTGATGGTGAAGGTAAAATCTGTCCCAGATCGACTTCC11870.12251246541112892No Hit
GTCTCCATCCTCCCTGTCTGCTTCTGTAGGAGACAGAGTCATCATCACTTGCCAGGCGAGTCAGGCCATCAACA11370.11735187293382779No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11270.11631975443836756No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11180.11539084779245337No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11120.11477157669517722No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG10900.11250091600516472No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA10840.11188164490788857No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10660.11002383161606018No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG10620.10961098421787609No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC10600.10940456051878404No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10500.1083724420233238No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10430.10764995907650166No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10410.10744353537740961No Hit
CTTTTGGCCAGGGGACCAGGCTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG10360.1069274761296795No Hit
ATATTACTGTCAGCAGTATTATAGTCTCCCGTACACTTTTGGCCAGGGGA10260.10589535763421928No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9780.10094118885601018No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCC9750.1006315533073721No Hit
GTAATATGTTGCAACATCTTCAGGCTGCAGGTTGCTGATGGTGAAGGTAAAATCTGTCCCAGATCGACTTCC9740.10052834145782609No Hit
CCTCATACCTGAGGACACGGGTCTCTATTACTGTGCGAGAGGGCGGCCTACTATGCCAGTAGAAGTGATCCTCG9730.10042512960828007No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)