FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968881
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATACTGCTGACAGTAATATGTTGCAACATCTTCAGGCTGCAGGTTGCTGATGGTGAAGGTAAAATCTGTC32640.336883476918218No Hit
GTATTATAGTCTCCCGTACACTTTTGGCCAGGGGACCAGGCTGGAAATCAAACGAACTGTGGCTGCACCAT30260.31231905672626464No Hit
GTAATAGAGACCCGTGTCCTCAGGTATGAGGTCGTCGATTTCAAGAGATAATGTATTCTTCAAATTGTCTCTGA22850.2358390762126618No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19980.20621727539295331No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT18690.19290294680151637No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG18310.1889808965187675No Hit
CTCTATTACTGTGCGAGAGGGCGGCCTACTATGCCAGTAGAAGTGATCCT16900.17442802573277835No Hit
CTATTACTGTGCGAGAGGGCGGCCTACTATGCCAGTAGAAGTGATCCTCG15100.15584989281449424No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15080.1556434691154022No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14240.1469736737535363No Hit
CAGTAATAGAGACCCGTGTCCTCAGGTATGAGGTCGTCGATTTCAAGAGATAATGTATTCTTCAAATTGTCTCTG13690.14129702202850505No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13650.14088417463032096No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGATTCAGCTTCAGTTATTACGCT13550.13985205613486074No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG13520.13954242058622268No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13370.13799424284303233No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA13300.13727175989621015No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13110.13531073475483574No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12620.1302533541270806No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCGTAATAACTGAAGCTGAATCCAGAGGCTGCAC12150.12540239719841756No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCATCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTG12000.1238542194552272No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT11850.12230604171203688No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11710.12086107581839255No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11670.12044822842020846No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11550.11920968622565618No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG11390.11755829663291983No Hit
GTCTCCATCCTCCCTGTCTGCTTCTGTAGGAGACAGAGTCATCATCACTTGCCAGGCGAGTCAGGCCATCAACA11040.11394588189880903No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10980.1133266108015329No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10710.11053989086379028No Hit
CAGTAATATGTTGCAACATCTTCAGGCTGCAGGTTGCTGATGGTGAAGGTAAAATCTGTCCCAGATCGACTTCC10580.109198136819692No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10510.10847565387286985No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC10270.10599856948376529No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG10200.10527608653694313No Hit
GGTATGAGGTCGTCGATTTCAAGAGATAATGTATTCTTCAAATTGTCTCT10190.10517287468739711No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10060.10383112064329882No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG9710.10021870590918801No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)