FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397527
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGAGTTCTTGGCGTTGTCTCTGG33990.24321533680565743No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT28880.20665074807141473No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA24690.1766692164086991No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC24100.1724474732867415No Hit
CCCCTGTGCTGGTCATCTATCAAGATAGCAAGCGGCCCTCAGGGATCCCT24090.17237591831857274No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC22310.15963913398453125No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC21720.15541739086257367No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATA20940.14983610334540942No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCCAGTGCTGCTGTCCCACGCCTGACAGTAATAGTC20920.14969299340907188No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20840.1491205536637217No Hit
GCACAGGGGACTGGCCTGGCTTCTGCTGATACCAGCAAGCATATTTATCC20170.14432637079641394No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG19830.1418935018786757No Hit
GTGTATTACTGTGCGAGGGATCTGACCCCAGCTATGGTTACGGGTGGTGGGGCCTACGGTATGGACGTCTGGGGC19250.13774331372488688No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC18650.13345001563476053No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATC18040.12908516257646543No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTC17840.12765406321308997No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCCAGTGCTGCTGTCCCACGCCTGACAGTAATAGTCAGC17620.12607985391337698No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCACTGGGGTCTTCGGAACTGGGACCAAGGT17220.1232176551866261No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG16550.11842347231931835No Hit
CCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGATAAATATGCTTGCTGGTATCAGCAGA16170.115704383528905No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGG16020.1146310590063734No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG15890.11370084442017936No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15830.11327151461116673No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCC15460.11062398078892215No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGT15050.10769022709400247No Hit
GACTATTACTGTCAGGCGTGGGACAGCAGCACTGGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTC14860.10633068269879581No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14770.10568668798527683No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGG14690.10511424823992667No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTC14470.10354003894021367No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT14420.10318226409936981No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)