FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219777
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30970.2538988683997157No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT29880.24496280877570245No Hit
GTAATAGACGGCCGTGTCTGCGGCTGTCACAGAGTTCAGGTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTG27000.22135193564069497No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC25410.20831676609740962No Hit
GTCTCCAGGCAGCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTTAGT23700.19429781017349892No Hit
CTCCTGTAGGGCCAGTCAGAGTGTTAGTAGAAACTACTTACTCTGGTACC23400.19183834422193566No Hit
TCACTAGACTGGAGCCTGAAGATTTTGGAGTCTATTACTGTCAGCAGTATGGTAGCTTCACTTTCGGCCCTGGG22490.18437796416886038No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21190.17372027837875284No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG19950.16355448577895795No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19710.16158691301770733No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG18190.14912561886312006No Hit
GTATGGTAGCTTCACTTTCGGCCCTGGGACCAAAGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT18080.1482238146808802No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17330.142075149801972No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA16980.1392057728584815No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16740.1372382000972309No Hit
GTCTATTACTGTGCCAGGACGCCACCTATTACCATGATTCTGAAGGGTTT16710.13699225350207456No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT16530.1355165739311366No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC16290.13354900116988597No Hit
GTCTAGTGATGGTGAGAGTGAAGTCTGTCCGAGACCCACTGGCACTGAAC15560.12756430068774866No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15530.12731835409259235No Hit
CAGTAATAGACTCCAAAATCTTCAGGCTCCAGTCTAGTGATGGTGAGAGTGAAGTCTGTCCGAGACCCACTGGC15170.1243669949507164No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCCCAGGGCCGAAAGTGAAGCTACCATACTGCTGACAGTAAT15030.12321924417332021No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT14580.11953004524597528No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGG14520.11903815205566264No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC14220.11657868610409935No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG14210.11649670390571391No Hit
CTATTACTGTCAGCAGTATGGTAGCTTCACTTTCGGCCCTGGGACCAAAGTGGAGATCAAACGAACTGTGGCTGC14060.11526697092993227No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC13920.11411922015253607No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13920.11411922015253607No Hit
GTCTCGGACAGACTTCACTCTCACCATCACTAGACTGGAGCCTGAAGATT13640.11182371859774368No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13350.10944623484456584No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA13150.107806590876857No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12920.1059210003139918No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12920.1059210003139918No Hit
GTGTTAGTAGAAACTACTTACTCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCGATGGTGCAT12580.10313360556888677No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12570.10305162337050132No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12520.10264171237857411No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12500.10247774798180323No Hit
CTACAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGCTGCCTGG12490.10239576578341777No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCCCAGGGCCGAAAGTGAAGCTACCAT12430.10190387259310513No Hit
GACTTCACTCTCACCATCACTAGACTGGAGCCTGAAGATTTTGGAGTCTATTACTGTCAGCAGTATGGTAGCTTC12240.10034621082378173No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12240.10034621082378173No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12200.10001828203023996No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)