FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219777
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30210.24766822132242206No Hit
GTAATAGACGGCCGTGTCTGCGGCTGTCACAGAGTTCAGGTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTG25390.2081528017006387No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT25070.20552937135230454No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC24120.1977410625056875No Hit
CTCCTGTAGGGCCAGTCAGAGTGTTAGTAGAAACTACTTACTCTGGTACC23020.18872302068328883No Hit
GTCTCCAGGCAGCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTTAGT22710.1861815725333401No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21020.17232658100620032No Hit
TCACTAGACTGGAGCCTGAAGATTTTGGAGTCTATTACTGTCAGCAGTATGGTAGCTTCACTTTCGGCCCTGGG19650.1610950198273947No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA18220.1493715654582764No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG17910.14683011730832765No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17830.14617425972124412No Hit
GTATGGTAGCTTCACTTTCGGCCCTGGGACCAAAGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT16870.13830396867624165No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16700.1369102713036891No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16540.13559855612952204No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC16140.13231926819410433No Hit
GTCTATTACTGTGCCAGGACGCCACCTATTACCATGATTCTGAAGGGTTT15710.1287940336635303No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT15020.12313726197493477No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCCCAGGGCCGAAAGTGAAGCTACCATACTGCTGACAGTAAT14700.12051383162660059No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT14660.12018590283305883No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14500.11887418765889174No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG14490.1187922054605063No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13970.1145291311444633No Hit
CAGTAATAGACTCCAAAATCTTCAGGCTCCAGTCTAGTGATGGTGAGAGTGAAGTCTGTCCGAGACCCACTGGC13760.112807504978369No Hit
GTCTAGTGATGGTGAGAGTGAAGTCTGTCCGAGACCCACTGGCACTGAAC13560.11116786101066013No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13480.11051200342357662No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC13280.10887235945586775No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13150.107806590876857No Hit
GTCTCGGACAGACTTCACTCTCACCATCACTAGACTGGAGCCTGAAGATT13010.1066588400994608No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12890.1056750537188355No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC12860.10542910712367916No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG12580.10313360556888677No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)