FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486214
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCGTGCTCCTGGAATTGTCTCTGG24230.498340237014977No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCC14740.30315869144039453No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12030.24742191709823247No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10910.2243867926468592No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10450.21492593796147375No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9950.20464240025996785No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9830.20217435121160643No Hit
GTATATTACTGTGCGAAAGATCTAAAGGTGATCACACAATATTACTTTGACTCCTGGGGCCAGGGAGCCCTGGTC9640.19826660688503417No Hit
AAGTAATATTGTGTGATCACCTTTAGATCTTTCGCACAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTC9480.19497587482055226No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTTGCCAACTATGCCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG9410.19353617954234145No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT9360.19250782577219086No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC9030.18572069088919693No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8880.18263562957874518No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT8790.18078459279247408No Hit
ACCACATACTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAGAGACAATTCCAGGAGCACGCTGTATCTG8530.17543715318769101No Hit
ATATTACTTTGACTCCTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTT8350.1717350796151489No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG8020.16494794473215496No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7770.159806175881402No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC7760.1596005051273719No Hit
CCTTTAGATCTTTCGCACAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCGTG7730.15898349286528154No Hit
GTATGTGGTACCACTACTACCACTTATACTTGAGACCCACTGCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT7590.1561041023088599No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGCTCCCTGG7590.1561041023088599No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7450.1532247117524382No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG7380.1517850164742274No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7210.14828861365571538No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7200.14808294290168525No Hit
GATCTATGCTGCGTCCCGTTTGCAAGCTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTC7050.14499788159123347No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG6910.14211849103481183No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6860.14109013726466124No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT6810.14006178349451065No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6730.1384164174622697No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6730.1384164174622697No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC6720.13821074670823957No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAACTGTTGGAGT6720.13821074670823957No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATC6720.13821074670823957No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC6690.13759373444614922No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG6620.13615403916793842No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6430.13224629484136616No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG6370.13101227031718543No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC6260.12874989202285414No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT6240.1283385505147939No Hit
GAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAAC6130.1260761722204626No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT6130.1260761722204626No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC6120.12587050146643247No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA6070.12484214769628188No Hit
CCCTGAGACTCTCCTGTGTAGCCTCTGGATTCACCTTTGCCAACTATGCC6040.12422513543419154No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGCTCCCTGG6030.1240194646801614No Hit
CTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGAGAACTCACC6030.1240194646801614No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG5910.1215514156318No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG5870.12072873261567951No Hit
GCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTG5850.12031739110761928No Hit
GTGTAGCCTCTGGATTCACCTTTGCCAACTATGCCATGAACTGGGTCCGC5600.11517562225686633No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT5590.1149699515028362No Hit
CTCCGTGAGGGGCCGGTTCACCATCTCCAGAGACAATTCCAGGAGCACGCTGTATCTGCAAATGAACAGCCTG5340.10982818265208324No Hit
GGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGCCTCTGGATTCACCTTTGCCAACTATGCCATG5320.10941684114402299No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGCCTCTGGATTCACCTTTGCCAACTATGCCATGAACTGGGTCCG5320.10941684114402299No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5290.10879982888193265No Hit
CTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGAGAACTC5250.10797714586581217No Hit
ATACTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAGAGACAATT5130.10550909681745074No Hit
GTATAAGTGGTAGTAGTGGTACCACATACTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAGAGACAATT5100.1048920845553604No Hit
GCCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG5090.10468641380133029No Hit
GTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCC5090.10468641380133029No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5050.1038637307852098No Hit
GTGTGAGGTGCAACTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGC5050.1038637307852098No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACGTTGGTCCCTCCGCCGAAAGTG5010.10304104776908934No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG5000.10283537701505921No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC4970.10221836475296886No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCG4960.10201269399893874No Hit
GCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAAC4920.10119001098281827No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG4920.10119001098281827No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGGTCG4890.10057299872072792No Hit
GTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTC4870.10016165721266766No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)