FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486214
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCGTGCTCCTGGAATTGTCTCTGG22320.4590571229952243No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCC12150.2498899661465939No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9470.19477020406652215No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTTGCCAACTATGCCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG9240.19003977672382943No Hit
ATATTACTTTGACTCCTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTT9230.1898341059697993No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8960.18428099561098613No Hit
GTATATTACTGTGCGAAAGATCTAAAGGTGATCACACAATATTACTTTGACTCCTGGGGCCAGGGAGCCCTGGTC8950.184075324856956No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8690.17872788525217292No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8630.1774938607279922No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8480.17440879941754042No Hit
ACCACATACTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAGAGACAATTCCAGGAGCACGCTGTATCTG8270.17008971358290795No Hit
AAGTAATATTGTGTGATCACCTTTAGATCTTTCGCACAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTC8260.1698840428288778No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8200.1686500183046971No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8070.16597629850230558No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT8040.1653592862402152No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8030.1651536154861851No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC7990.16433093247006464No Hit
CCTTTAGATCTTTCGCACAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCGTG7560.15548709004676953No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT7330.1507566627040768No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7180.14767160139362503No Hit
GATCTATGCTGCGTCCCGTTTGCAAGCTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTC6980.14355818631302267No Hit
GTATGTGGTACCACTACTACCACTTATACTTGAGACCCACTGCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT6980.14355818631302267No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG6900.14191282028078173No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG6780.1394447712324203No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGCTCCCTGG6540.13450867313569745No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC6380.13121794107121557No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC6210.12772153825270355No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC6120.12587050146643247No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATC6080.125047818450312No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6010.12360812317210117No Hit
CTCCGTGAGGGGCCGGTTCACCATCTCCAGAGACAATTCCAGGAGCACGCTGTATCTGCAAATGAACAGCCTG5790.11908336658343857No Hit
CCCTGAGACTCTCCTGTGTAGCCTCTGGATTCACCTTTGCCAACTATGCC5780.11887769582940846No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG5780.11887769582940846No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA5750.11826068356731809No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG5720.11764367130522775No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5720.11764367130522775No Hit
GCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTG5700.1172323297971675No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGCTCCCTGG5690.11702665904313739No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC5660.11640964678104704No Hit
GAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAAC5590.1149699515028362No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG5530.11373592697865549No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5520.11353025622462536No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAACTGTTGGAGT5490.11291324396253502No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA5490.11291324396253502No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT5400.11106220717626396No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT5390.11085653642223382No Hit
GTGTAGCCTCTGGATTCACCTTTGCCAACTATGCCATGAACTGGGTCCGC5350.11003385340611337No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC5230.10756580435775194No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAA5180.10653745058760133No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG5140.10571476757148088No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5120.10530342606342062No Hit
GGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGCCTCTGGATTCACCTTTGCCAACTATGCCATG5110.10509775530939051No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGCCTCTGGATTCACCTTTGCCAACTATGCCATGAACTGGGTCCG5080.10448074304730016No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5060.10406940153923991No Hit
CTGCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATGGCATAGTTGGCAAAGGTGAATCCAGAGGCTACAC5010.10304104776908934No Hit
GGGCTCCCTGGCCCCAGGAGTCAAAGTAATATTGTGTGATCACCTTTAGA4950.10180702324490863No Hit
ATACTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAGAGACAATT4930.10139568173684838No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA4880.1003673279666978No Hit
CTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGAGAACTC4870.10016165721266766No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)