FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512057
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT15670.30602061879829784No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15650.3056300372810058No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTATC11340.22145972030457547No Hit
GTATCAACACAGACCTGGCCAGGGTCCCAGGCTCCTCATCTATGATGCATCAAACAGGGCCACTGGCATCCCAGC10360.20232122595726648No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG10150.19822012002570027No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG10150.19822012002570027No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10150.19822012002570027No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT9630.18806500057610773No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9580.1870885467828777No Hit
CTCCTGTAGGGCCAGTCAGAGTGTTGACACCTCCTTGGCCTGGTATCAACACAGACCTGGCCAGGGTCCCAGGCT8780.17146528609119688No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAACCTCAGCAGCACCCTGACGCTGAGCAA8730.17048883229796682No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGCGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTTGACAC8390.16384894650400247No Hit
ACACAGACCTGGCCAGGGTCCCAGGCTCCTCATCTATGATGCATCAAACAGGGCCACTGGCATCCCAGCCAGGTT8380.16365365574535648No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8250.16111487588295834No Hit
GTCCGGGACAGACTTCACTCTCACCATCAGCAACTTGGAACCTGAAGATT8230.16072429436566632No Hit
GTTTATATCTGTCAACAGCGTAGCCGCTGGGTCACTTTCGGCAGAGGGAC7900.154279699330348No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA7810.1525220825025339No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7700.15037388415742778No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC7530.1470539412604456No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCTCTGCCGAAAGTGACCCAGCGGCTACGCTGTTGAC7280.14217167229429536No Hit
GTGTTGATACCAGGCCAAGGAGGTGTCAACACTCTGACTGGCCCTACAGGAGAGGGTGGCTCTTTCGCCTGG7280.14217167229429536No Hit
GCGTCAGGGTGCTGCTGAGGTTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG6990.13650824029356107No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6910.13494591422439298No Hit
GATATAAACTGCAAAATCTTCAGGTTCCAAGTTGCTGATGGTGAGAGTGA6900.13475062346574698No Hit
GACTTCACTCTCACCATCAGCAACTTGGAACCTGAAGATTTTGCAGTTTA6570.12830602843042865No Hit
GGTTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC6520.1273295746371986No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC6420.1253766670507385No Hit
GTCCCAGGCTCCTCATCTATGATGCATCAAACAGGGCCACTGGCATCCCA6400.12498608553344646No Hit
GTCCCGGACCCACTGCCAGTGAACCTGGCTGGGATGCCAGTGGCCCTGTT6260.12225201491240234No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6250.12205672415375633No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT6240.12186143339511031No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC6160.12029910732594222No Hit
GTCTTTGTCTCCAGGCGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGA6040.1179556182221901No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5930.11580741987708398No Hit
GACCTGGCCAGGGTCCCAGGCTCCTCATCTATGATGCATCAAACAGGGCC5910.11541683835979198No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5840.11404980304926991No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5710.11151102318687177No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCTCTGCCGAAAGTGACCCAGCGGCT5670.11072986015228774No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5420.10584759118613747No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC5260.10272293904780132No Hit
GAGTGAAGTCTGTCCCGGACCCACTGCCAGTGAACCTGGCTGGGATGCCAGTGGCCCTGTTTGATGCATCAT5190.10135590373727923No Hit
CATCTATGATGCATCAAACAGGGCCACTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTCCGGGACAGACTTC5140.1003794499440492No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG5140.1003794499440492No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT5130.10018415918540317No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)