FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512057
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15220.2972325346592274No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT13240.25856496444731736No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGTATC9880.192947269542258No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9780.1909943619557979No Hit
GTATCAACACAGACCTGGCCAGGGTCCCAGGCTCCTCATCTATGATGCATCAAACAGGGCCACTGGCATCCCAGC9730.19001790816256783No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAACCTCAGCAGCACCCTGACGCTGAGCAA9400.1835733131272495No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9100.17771459036786919No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG8620.16834063395286072No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8610.1681453431942147No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT8560.16716888940098465No Hit
GTCCGGGACAGACTTCACTCTCACCATCAGCAACTTGGAACCTGAAGATT8380.16365365574535648No Hit
CTCCTGTAGGGCCAGTCAGAGTGTTGACACCTCCTTGGCCTGGTATCAACACAGACCTGGCCAGGGTCCCAGGCT7830.15291266401982592No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7760.15154562870930385No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGCGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTTGACAC7670.14978801188148977No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG7470.14588219670856956No Hit
GTTTATATCTGTCAACAGCGTAGCCGCTGGGTCACTTTCGGCAGAGGGAC7440.14529632443263152No Hit
ACACAGACCTGGCCAGGGTCCCAGGCTCCTCATCTATGATGCATCAAACAGGGCCACTGGCATCCCAGCCAGGTT7230.1411952185010653No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7170.14002347394918924No Hit
GTGTTGATACCAGGCCAAGGAGGTGTCAACACTCTGACTGGCCCTACAGGAGAGGGTGGCTCTTTCGCCTGG6910.13494591422439298No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC6740.1316259713274108No Hit
GCGTCAGGGTGCTGCTGAGGTTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG6670.13025893601688873No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCTCTGCCGAAAGTGACCCAGCGGCTACGCTGTTGAC6620.12928248222365868No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6560.12811073767178263No Hit
GAGCAGGACAGCAAGGACAGCACCTACAACCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC6550.1279154469131366No Hit
GATATAAACTGCAAAATCTTCAGGTTCCAAGTTGCTGATGGTGAGAGTGA6290.12283788718834036No Hit
GTCCCAGGCTCCTCATCTATGATGCATCAAACAGGGCCACTGGCATCCCA6190.12088497960188027No Hit
GGTTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC6150.12010381656729621No Hit
GTCTTTGTCTCCAGGCGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGA6130.1197132350500042No Hit
GACTTCACTCTCACCATCAGCAACTTGGAACCTGAAGATTTTGCAGTTTA6020.1175650367048981No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA5940.11600271063573No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT5810.11346393077333186No Hit
GACCTGGCCAGGGTCCCAGGCTCCTCATCTATGATGCATCAAACAGGGCC5720.11170631394551778No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCTCTGCCGAAAGTGACCCAGCGGCT5650.11033927863499571No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT5640.11014398787634969No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5540.1081910802898896No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT5440.1062381727034295No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC5370.10487113739290743No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5370.10487113739290743No Hit
GTCCCGGACCCACTGCCAGTGAACCTGGCTGGGATGCCAGTGGCCCTGTT5330.10408997435832339No Hit
CCTACAACCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC5170.10096532221998723No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)