FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598411
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20480.34223969813389127No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT18240.3048072311504969No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGAGACAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATATTAGA16240.27138538562960907No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGTCTCAGGGAGAATTGGTTCTTGGACAGGTCAACTG16080.268711637987938No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACTG16050.26821031030512477No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14930.24949407681342756No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14350.23980174161237008No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG13400.22392636498994836No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12290.20537724072585564No Hit
GTCTCTGGTGGCTCCGTCAGCAGTGCCGATTACTTCTGGAGTTGGATCCG12140.20287060231178908No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG11810.19735599780084256No Hit
CTCCTGCAGGGCCAGTCAGAATATTAGAAGATTCTTGGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT11740.19618623320761147No Hit
ACCTAGAGCCTGGAGATTTTGCAGTTTATTTCTGTCACCAACGCAGCAGC11670.19501646861438043No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG11520.19250983020031384No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC10760.17980952890237645No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT10620.17746999971591432No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10530.17596601666747436No Hit
GACTTCACTCTCACCATCAGCAACCTAGAGCCTGGAGATTTTGCAGTTTATTTCTGTCACCAACGCAGCAGCTGG10280.17178828597736337No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG10120.16911453833569234No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC10000.16710922760443908No Hit
CACCTGCACTGTCTCTGGTGGCTCCGTCAGCAGTGCCGATTACTTCTGGAGTTGGATCCGTCAGCCCCCAGGGA9950.16627368146641688No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGAGGCC9630.16092618618307483No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC9610.16059196772786596No Hit
GTCTTTGTCTCCAGGAGACAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATATTAGAAGATTCTTGGCCTGGT9260.15474314476171058No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA9080.1517351786648307No Hit
TCTATGATGCATCCAACAGGGCCGCTGGCATCCCAGCCAGGTTCAATGGCAGTGGGTCTGGGACGGACTTCACT8890.14856010334034636No Hit
GTCTGGGACGGACTTCACTCTCACCATCAGCAACCTAGAGCCTGGAGATTTTGCAGTTTATTTCTGTCACCAACG8800.1470561202919064No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8800.1470561202919064No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG8470.14154151578095991No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8420.14070596964293772No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA8240.13769800354605782No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8180.13669534818043116No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8070.13485714667678234No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7980.1333531636283424No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7900.1320162898075069No Hit
CATCTATGATGCATCCAACAGGGCCGCTGGCATCCCAGCCAGGTTCAATGGCAGTGGGTCTGGGACGGACTTC7690.12850699602781365No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7670.12817277757260478No Hit
GTGTATTACTGTGCCAGAGTGGGGTACTGCAGTGGCGGTAACTGCTACGG7540.1260003576137471No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGAGGCCAGCTGCTGCGTTGGTGA7370.12315950074447161No Hit
CTATAATGGGTGTTCCCAATCCACTCCAGGGCCTTCCCTGGGGGCTGACGGATCCAACTCCAGAAGTAATCGGC7260.12132129924082279No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT7250.12115419001321834No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGA7240.1209870807856139No Hit
ACATACAACAACCCGTCCCTCAAGAGTCGCCTTGTCATGTCAGTTGACCTGTCCAAGAACCAATTCTCCCTG7240.1209870807856139No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7230.12081997155800946No Hit
GTTGTATGTGGTCCCACTATAATGGGTGTTCCCAATCCACTCCAGGGCCTTCCCTGGGGGCTGACGGATCCAACT7220.12065286233040502No Hit
GAGTGAAGTCCGTCCCAGACCCACTGCCATTGAACCTGGCTGGGATGCCAGCGGCCCTGTTGGATGCATCAT7170.11981731619238284No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC7100.11864755159915175No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCGCTGGCATCCCAGCCAGGTT7070.11814622391633843No Hit
CCCAGATACTGCCGGAGAAATTGTTTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGAGACAGAGCCAC7060.11797911468873398No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7010.1171435685507118No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC6990.11680935009550292No Hit
GTTCAATGGCAGTGGGTCTGGGACGGACTTCACTCTCACCATCAGCAACC6930.11580669472987627No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6860.11463693013664522No Hit
CCCATTATAGTGGGACCACATACAACAACCCGTCCCTCAAGAGTCGCCTTGTCATGTCAGTTGACCTGTCCAAGA6820.11396849322622746No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT6770.11313294708820527No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC6740.11263161940539194No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6730.11246451017778751No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC6720.11229740095018306No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6700.11196318249497418No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6690.11179607326736975No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGC6610.11045919944653423No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAT6540.10928943485330317No Hit
CTGCTACTCTGGCTCCCAGATACTGCCGGAGAAATTGTTTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCC6510.10878810717048985No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAATATTAGAAGATTCTTGGCCTGGTACCAACAGAAACCTGGCCAGG6500.1086209979428854No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6470.10811967026007209No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCGCTGGCATCCCA6460.10795256103246764No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT6440.10761834257725876No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCTGGGTTCCCTGGCCCCAGTCGTCAAAGTAGTAATC6360.10628146875642326No Hit
GGCCGAAGGTGATCGGAGGCCAGCTGCTGCGTTGGTGACAGAAATAAACTGCAAAATCTCCAGGCTCTAGGTTG6320.1056130318460055No Hit
GTCTCAGGGAGAATTGGTTCTTGGACAGGTCAACTGACATGACAAGGCGA6260.10461037648037887No Hit
GTCCCAGACCCACTGCCATTGAACCTGGCTGGGATGCCAGCGGCCCTGTT6200.10360772111475222No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)