FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598411
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20890.3490911764656733No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGAGACAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATATTAGA15350.256512664372814No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT15080.2520007152274942No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACTG14570.24347814461966777No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGTCTCAGGGAGAATTGGTTCTTGGACAGGTCAACTG13360.22325792807953065No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12440.2078838791399222No Hit
GTCTCTGGTGGCTCCGTCAGCAGTGCCGATTACTTCTGGAGTTGGATCCG12170.20337192999460235No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12120.20253638385658018No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA12090.20203505617376688No Hit
CTCCTGCAGGGCCAGTCAGAATATTAGAAGATTCTTGGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT12020.2008652915805358No Hit
ACCTAGAGCCTGGAGATTTTGCAGTTTATTTCTGTCACCAACGCAGCAGC11420.19083873792426945No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11420.19083873792426945No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC10680.17847265508154095No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG10110.16894742910808794No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG9950.16627368146641688No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC9880.16510391687318582No Hit
CACCTGCACTGTCTCTGGTGGCTCCGTCAGCAGTGCCGATTACTTCTGGAGTTGGATCCGTCAGCCCCCAGGGA9880.16510391687318582No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT9470.15825243854140383No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9180.15340627094087508No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG9150.15290494325806175No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9090.15190228789243512No Hit
CTGCTACTCTGGCTCCCAGATACTGCCGGAGAAATTGTTTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCC9050.15123385098201736No Hit
GTCTTTGTCTCCAGGAGACAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATATTAGAAGATTCTTGGCCTGGT8970.14989697716118186No Hit
GACTTCACTCTCACCATCAGCAACCTAGAGCCTGGAGATTTTGCAGTTTATTTCTGTCACCAACGCAGCAGCTGG8920.14906143102315966No Hit
TCTATGATGCATCCAACAGGGCCGCTGGCATCCCAGCCAGGTTCAATGGCAGTGGGTCTGGGACGGACTTCACT8470.14154151578095991No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8390.14020464196012442No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG8060.1346900374491779No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC8040.134355818993969No Hit
GTCTGGGACGGACTTCACTCTCACCATCAGCAACCTAGAGCCTGGAGATTTTGCAGTTTATTTCTGTCACCAACG7790.13017808830385805No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGAGGCC7740.12934254216583585No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7690.12850699602781365No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC7640.12767144988979148No Hit
ACATACAACAACCCGTCCCTCAAGAGTCGCCTTGTCATGTCAGTTGACCTGTCCAAGAACCAATTCTCCCTG7420.12399504688249381No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC7380.12332660997207605No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7370.12315950074447161No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC7360.12299239151686717No Hit
CATCTATGATGCATCCAACAGGGCCGCTGGCATCCCAGCCAGGTTCAATGGCAGTGGGTCTGGGACGGACTTC7300.12198973615124052No Hit
GTGTATTACTGTGCCAGAGTGGGGTACTGCAGTGGCGGTAACTGCTACGG7260.12132129924082279No Hit
GGCCGAAGGTGATCGGAGGCCAGCTGCTGCGTTGGTGACAGAAATAAACTGCAAAATCTCCAGGCTCTAGGTTG7100.11864755159915175No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG7090.11848044237154731No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGAGGCCAGCTGCTGCGTTGGTGA7050.11781200546112956No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6910.11547247627466742No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6910.11547247627466742No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT6840.11430271168143633No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6830.11413560245383189No Hit
GAGTGAAGTCCGTCCCAGACCCACTGCCATTGAACCTGGCTGGGATGCCAGCGGCCCTGTTGGATGCATCAT6800.11363427477101859No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6720.11229740095018306No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6680.11162896403976533No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC6660.11129474558455645No Hit
GTTGTATGTGGTCCCACTATAATGGGTGTTCCCAATCCACTCCAGGGCCTTCCCTGGGGGCTGACGGATCCAACT6650.111127636356952No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6640.11096052712934756No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG6570.10979076253611647No Hit
CCCAGATACTGCCGGAGAAATTGTTTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGAGACAGAGCCAC6500.1086209979428854No Hit
CTATAATGGGTGTTCCCAATCCACTCCAGGGCCTTCCCTGGGGGCTGACGGATCCAACTCCAGAAGTAATCGGC6420.1072841241220499No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCGCTGGCATCCCAGCCAGGTT6410.10711701489444546No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA6370.1064485779840277No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6370.1064485779840277No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT6270.10477748570798331No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC6170.10310639343193892No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC6160.10293928420433449No Hit
GTTCAATGGCAGTGGGTCTGGGACGGACTTCACTCTCACCATCAGCAACC6110.10210373806631227No Hit
GTCCCAGACCCACTGCCATTGAACCTGGCTGGGATGCCAGCGGCCCTGTT6030.10076686424547678No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6030.10076686424547678No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCTGGGTTCCCTGGCCCCAGTCGTCAAAGTAGTAATC6000.10026553656266345No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)