FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694190
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAACACACAACGTTCGGCCAAGGGACCAAGGTGGAAATTAAACGAACTGTGGCTGCACCAT19650.2830637145450093No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19340.2785980783359023No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT18550.26721790864172634No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGTA17500.25209236664313805No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG14150.20383468502859448No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13400.1930307264581743No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12940.18640429853498322No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG12670.18251487344963194No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG12410.1787695011452196No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12340.17776113167864707No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGAGAACGAGCCACTCTCTCGT12210.1758884455264409No Hit
CAATAATACACTGCAAAATCTTCAGACTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC12000.17286333712672322No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC11750.16926201760324985No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGTCTGAAGATTTTGCAGTGTATTATTGTCAACACAC11550.1663809619844711No Hit
TAACTACACTGTATCGCTCTGGCACAGAAATACACGGCAGTGTCGTCAGC11340.16335585358475346No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG10370.14938273383367667No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10360.14923868105273772No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT10200.14693383655771475No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9640.13886688082513432No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC9380.135121508520722No Hit
GTGCAGCCACAGTTCGTTTAATTTCCACCTTGGTCCCTTGGCCGAACGTTGTGTGTTGACAATAATACACTGCAA9370.13497745573978306No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTCATTGGCCCTACT9350.1346893501779052No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGAGAACGAGCCACTCTCTCGTGT9190.1323845056828822No Hit
GCTCCACACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAGCAACAGGTGCCCACTCCCAGGTGC9070.130655872311615No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC9040.13022371396879817No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA8960.12907129172128667No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG8770.1263342888834469No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8510.12258891657903456No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA8280.11927570261743904No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8190.1179792275889886No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC8170.11769112202711073No Hit
GTGTATTATTGTCAACACACAACGTTCGGCCAAGGGACCAAGGTGGAAATTAAACGAACTGTGGCTGCACCAT8010.11538627753208777No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7900.11380169694175946No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGTCTGAAGATTTTGCAGTGTATTATTGTCAACACACAACGTTCGGC7600.10948011351359138No Hit
ATAATACACTGCAAAATCTTCAGACTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC7520.1083276912660799No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATTTCCACCTTGGTCCCTTGGCCGAACGTTGTGTGTTGACA7360.10602284677105692No Hit
GAATAGCAGTTACCATAACTACACTGTATCGCTCTGGCACAGAAATACAC7270.10472637174260649No Hit
GTCTTTGTCTCCAGGAGAACGAGCCACTCTCTCGTGTAGGGCCAGTCAGA7190.10357394949509499No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT7140.10285368559040033No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6980.10054884109537736No Hit
GTATCGCTCTGGCACAGAAATACACGGCAGTGTCGTCAGCCTTTAGATTGTTGATCTGCAGATATGCCATATTG6970.10040478831443841No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)