FastQCFastQC Report
Wed 8 May 2024
EGAF00004717946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694190
Total Bases51.1 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21410.30841700399026206No Hit
GTATTATTGTCAACACACAACGTTCGGCCAAGGGACCAAGGTGGAAATTA21220.3056800011524223No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC16700.24056814416802316No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16130.23235713565450383No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG13810.19893689047667065No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGTCTGAAGATT13500.19447125426756362No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13150.18942940693470087No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12870.18539592906841065No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12840.18496377072559386No Hit
CAATAATACACTGCAAAATCTTCAGACTCCAGTCTGCTGATGGTGAGAGT12280.17689681499301343No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12180.17545628718362408No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12150.17502412884080726No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG12110.1744479177170515No Hit
TAACTACACTGTATCGCTCTGGCACAGAAATACACGGCAGTGTCGTCAGC11180.16105100908973047No Hit
GTGCAGCCACAGTTCGTTTAATTTCCACCTTGGTCCCTTGGCCGAACGTT11140.16047479796597472No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGAGAACGAGCCACTCTCTCGT10960.1578818479090739No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG10960.1578818479090739No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10650.15341621169996686No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTCATTGGCCCTACT10440.1503911033002492No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10360.14923868105273772No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA10300.1483743643671041No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10020.1443408865008139No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT9820.14145983088203518No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGAGAACGAGCCACTCTCTCGTGT9490.13670608911105028No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9320.13425719183508836No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC9310.13411313905414946No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9290.13382503349227157No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC9210.13267261124476007No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT9130.1315201889972486No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA8910.128351027816592No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8820.12705455278814157No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8720.12561402497875224No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATTTCCACCTTGGTCC8650.12460565551217966No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC8550.12316512770279031No Hit
ATAATACACTGCAAAATCTTCAGACTCCAGTCTGCTGATGGTGAGAGTGA8500.12244486379809562No Hit
GTGTATTATTGTCAACACACAACGTTCGGCCAAGGGACCAAGGTGGAAAT8440.12158054711246201No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8400.12100433598870627No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT8400.12100433598870627No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGTCTGAAGATTTTGCAGTGTA8210.11826733315086646No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8000.11524222475114883No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC7970.11481006640833202No Hit
CTGCTACTCTGGCTCCCAGATAGTAACGGAGAAATTGTGTTGACGCAGTC7620.10976821907546924No Hit
GTATCGCTCTGGCACAGAAATACACGGCAGTGTCGTCAGCCTTTAGATTG7600.10948011351359138No Hit
GTCTTTGTCTCCAGGAGAACGAGCCACTCTCTCGTGTAGGGCCAGTCAGA7510.10818363848514095No Hit
GCTCCACACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAG7490.1078955329232631No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC7400.10659905789481267No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA7290.10501447730448436No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG7280.10487042452354542No Hit
CTCCCAGGTGCAGCTGGTGCAATCTGGGTCTGAGTTGAAGGAGGCTGGGG7150.10299773837133926No Hit
CTCTCGTGTAGGGCCAGTCAGACTGTTGGTTGGGGCTCTTTGGCCTGGTA7120.10256558002852245No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7040.10141315778101095No Hit
CTCGTGTAGGGCCAGTCAGACTGTTGGTTGGGGCTCTTTGGCCTGGTACC7030.10126910500007202No Hit
GTGTAGGGCCAGTCAGACTGTTGGTTGGGGCTCTTTGGCCTGGTACCAAC6960.10026073553349947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)