FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705286
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20930.29675904526674285No Hit
GTAATATACGGCCGTGTCCTCGGATCTCAGGCTGCTCAATTCCATGTAGGCTGTGCTCGTGGATTCGTCCGCGGT19730.27974467095617944No Hit
GTATTACTGTCAACAGCATGTCAGGTCACTTATGTATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA16040.2274254699511971No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT14100.19991889814911962No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC13730.1946727994033626No Hit
CTCCTGCAGGGCCAATCAGAGTGTTAGCAGCAGCTACTTTGCCTGGTACC12690.1799270083342077No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC12400.17581520120915486No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA12030.17056910246339782No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12010.17028552955822177No Hit
GTGTATTACTGTCAACAGCATGTCAGGTCACTTATGTATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA11790.16716622760128513No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT11470.16262906111846825No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11280.15993511851929573No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11020.156248670752007No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10740.1522786500795422No Hit
GTCTCCAGGCACCCTGTCTTTGTTTCCAGGTGAAAGAGCCACCCTCTCCTGCAGGGCCAATCAGAGTGTTAGC10690.15156971781660206No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC10600.1502936397433098No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGAT9920.14065216096732389No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9710.1376746454629753No Hit
CTCCAGGCACCCTGTCTTTGTTTCCAGGTGAAAGAGCCACCCTCTCCTGCAGGGCCAATCAGAGTGTTAGCAGC9500.13469712995862673No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9490.13455534350603868No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9470.13427177060086262No Hit
GTATATTACTGTGCGAGGAAACTACTCCTGTGGGAGCGAGAAGCTTTTGATATGTGGGGCCAGGGGACAATGGTC9340.13242854671721827No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9170.1300181770232218No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9090.12888388540251755No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9020.1278913802344014No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG8950.12689887506628517No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8920.12647351570852108No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG8890.126048156350757No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT8830.1251974376352288No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8790.12463029182487671No Hit
GTCCAGGGGCCTGTCGCACCCAGCTGATAGTATAGCCGCTGAAGGTGCCTCCAGAAACCTTGCAGGAGACCTTC8770.12434671891970066No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC8700.12335421375158447No Hit
GTACCAGCAGAGAGCTGGCCAGGCTCCCAGGCTCCTCATCTATGATACATCCATCAGGGCCACTGGCATCCC8520.12080205760499996No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG8430.11952597953170771No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGGTACAGCAAAGTACGCACAGAAATTAC8250.1169738233851232No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTA7890.11186951109195419No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG7880.11172772463936616No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7790.1104516465660739No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7700.10917556849278165No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7700.10917556849278165No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCCTGGCCCCACATATCAAAAGCTTCTCGCT7680.10889199558760558No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7490.10619805298843306No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGTTTCTGGAGGCACCTTCAGCGG7390.10478018846255278No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC7350.10421304265220066No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC7330.1039294697470246No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTGC7280.10322053748408448No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC7250.10279517812632039No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG7190.10194445941079222No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)