FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256813
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGT29010.23082192816274177No Hit
GTAATACAAGGCCGTGTCCTCAGGTCTCAGACTGTTCATTTGTAGATACAGGGAGTTCTTGGCGTTGTCTCTGG26610.21172600856292859No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26510.21093034524626975No Hit
GACCTAGGACGGTCAGCTTGGTCCCTACGCCGAATACCAGAGTGCTGCTGCTTGTATATGAGCTGCAGTAATAAC25870.2058381000196529No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTACGCCGAATACCAGAGTGCTGCTGCTTGTATATGAGCTG25210.20058672212970424No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG24510.19501707891309208No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC24500.1949375125814262No Hit
GTCTGAGACCTGAGGACACGGCCTTGTATTACTGTGCAAAACTATCACAG23800.18936786936481403No Hit
CTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTGCATGGCATAATCATCAAAGGTGAATCCAGAGGCTGCAC22880.1820477668515523No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22350.17783075127326023No Hit
GGTTATTACTGCAGCTCATATACAAGCAGCAGCACTCTGGTATTCGGCGTAGGGACCAAGCTGACCGTCCTAGGT22280.17727378695159898No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21900.17425026634829527No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21730.17289763870997515No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGT21630.17210197539331626No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA21540.17138587840832328No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20830.1657366688600452No Hit
AGCATAGGCTATGCGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCCCTGTATCT20660.16438404122172512No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19640.15626827539180452No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCT19060.15165342815518298No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18930.15061906584352644No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC18890.1503008005168629No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18730.14902773921020868No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGATGATTATGCCATGCACTGGGTCCGGCAAGCTCCAGGGAAGGGC18400.14640205026523437No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCC18120.1441741929785895No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGGTTATTACTGCAGCTCATAT18090.14393549398359184No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17990.14313983066693295No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17680.14067327438529043No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT17580.13987761106863153No Hit
GTAATAACCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAG17400.13844541709864555No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16730.13311447287703104No Hit
CAGTAATACAAGGCCGTGTCCTCAGGTCTCAGACTGTTCATTTGTAGATA16570.13184141157037682No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC16550.13168227890704506No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16520.1314435799120474No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16350.13009095227372727No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16290.12961355428373195No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAAC16230.12913615629373662No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15640.12444174272544921No Hit
GCCTATGCTACCGCTATTCCAACTAATACCTGAGACCCACTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTG15550.1237256457404562No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC15440.12285041609213146No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG15260.12141822212214547No Hit
GCTTGGTACAGCCTGGCAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTGATGATTATGCCATGC15230.1211795231271478No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15210.12102039046381602No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15180.12078169146881836No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG15140.1204634261421548No Hit
CTCCAGAGACAACGCCAAGAACTCCCTGTATCTACAAATGAACAGTCTGAGACCTGAGGACACGGCCTTGTATT14940.11887209950883704No Hit
CTCCAGGGAAGGGCCTGGAGTGGGTCTCAGGTATTAGTTGGAATAGCGGTAGCATAGGCTATGCGGACTCTGTGA14530.11560987991053563No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG14440.11489378292554263No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14340.11409811960888373No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGA14290.11370028795055431No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT14120.1123476603122342No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGT14120.1123476603122342No Hit
GTATTACTGTGCAAAACTATCACAGCCCTGGTCCTCATACTACTACTACGGTATGGACGTCTGGGGCCAAGGGAC14060.11187026232223887No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC14030.11163156332724121No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13930.11083590001058233No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC13870.110358502020587No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13810.10988110403059168No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG13800.10980153769892577No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC13790.10972197136725989No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13720.10916500704559867No Hit
GCCTTGTATTACTGTGCAAAACTATCACAGCCCTGGTCCTCATACTACTACTACGGTATGGACGTCTGGGGCCA13640.10852847639227156No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13550.10781237940727857No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAA13520.10757368041228091No Hit
GTCTCAGACTGTTCATTTGTAGATACAGGGAGTTCTTGGCGTTGTCTCTGGAGATGGTGAATCGGCCCTTCAC13490.10733498141728323No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGTTTCCATTCAGTGAT13490.10733498141728323No Hit
ATACAAGGCCGTGTCCTCAGGTCTCAGACTGTTCATTTGTAGATACAGGG13470.10717584875395145No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAAT13260.1055049557889678No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13090.10415232815064772No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13030.10367493016065238No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG13000.10343623116565472No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12960.10311796583899117No Hit
TTGTATTACTGTGCAAAACTATCACAGCCCTGGTCCTCATACTACTACTACGGTATGGACGTCTGGGGCCAAGGG12880.10248143518566406No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC12680.10089010855234629No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTGG12670.10081054222068041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)