FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485322
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGCCTCTCAGGCTGGTCATTTGCAGAAACAGCGAATTCTGGGCGTTGTCTCTGG16290.33565344245676065No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15700.3234965651670437No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT14800.30495217608103486No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11700.2410770581181154No Hit
CTATAAACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG11370.23427744878657883No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11340.23365930248371186No Hit
GTCTATTACTGTGCAAGGAGGAGGAAGTTTCTATATGATAGTAGTGGTCAGCCCGATTACTACGCTATGGACGT10790.22232662026448421No Hit
CATCTATTCTGTTCGGAATTTGTTGAGAGGCGTCCCACCAAGGTTCAGTGGCGGCGGCTCTGGGACTTATTTC10650.2194419375177717No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG10540.21717540107392616No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG10270.21161208434812348No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9890.20378223117847533No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9270.19100720758589143No Hit
GAATAGATGAGGAGTTTTGGGGCTCTCCCACGTACGTGTTGATACCAATT9220.18997696374777984No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT8600.17720194015519594No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8560.17637774508470666No Hit
GTAGTAGGTTCCACAATCCTCAGGCTGCAAATTGCTGATGGTGAGAGTGAAATAAGTCCCAGAGCCGCCGCC8460.17431725740848344No Hit
AGTCAGAACATTGGCGTCTATTTGAATTGGTATCAACACGTACGTGGGAGAGCCCCAAAACTCCTCATCTATT8370.17246281849988254No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGGCGT7570.15597891709009687No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA7520.15494867325198527No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC7490.1543305269491183No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7480.154124478181496No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA7440.1533002831110067No Hit
CTGCTACTCTGGCTCCGAGGGGCCAGATGTGACATCCAGATGACCCAGTC7390.1522700392728951No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA7320.15082769789953887No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7310.15062164913191653No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG7280.1500035028290496No Hit
GTGCAGCCACAGTTCGTTTGATGTTTATAGTGGTCCCTCCGCCGAAGGTGAGCGGGGAAGTGTGGCTCTGCTGAC7200.14835511268807103No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGGGCCAGATGTGACATCC7140.1471188200823371No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGGGCCAGATGTGACAT6840.14093735705366744No Hit
ACCTACTACTGTCAGCAGAGCCACACTTCCCCGCTCACCTTCGGCGGAGGGACCACTATAAACATCAAACGA6730.13867082060982194No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTTTATAGTGGTCC6730.13867082060982194No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC6690.13784662553933266No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6680.13764057677171032No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT6630.13661033293359873No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6560.13516799156024248No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGGCGTCTATTTGAATTGGTATCAACACGT6540.13475589402499782No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG6540.13475589402499782No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6350.13084096744017373No Hit
CAGTAGTAGGTTCCACAATCCTCAGGCTGCAAATTGCTGATGGTGAGAGTGAAATAAGTCCCAGAGCCGCCGCC6340.13063491867255142No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6330.13042886990492908No Hit
ATGTTTATAGTGGTCCCTCCGCCGAAGGTGAGCGGGGAAGTGTGGCTCTGCTGACAGTAGTAGGTTCCACAATC6260.12898652853157286No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6220.12816233346108358No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6040.1244534556438818No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6000.12362926057339252No Hit
GTGTGGCTCTGCTGACAGTAGTAGGTTCCACAATCCTCAGGCTGCAAATTGCTGATGGTGAGAGTGAAATAAGTC5940.12239296796765857No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5900.12156877289716929No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5810.11971433398856841No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC5790.11930223645332377No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5710.11765384631234521No Hit
CTACTACTGTCAGCAGAGCCACACTTCCCCGCTCACCTTCGGCGGAGGGA5690.11724174877710057No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC5670.11682965124185593No Hit
GAGCCACACTTCCCCGCTCACCTTCGGCGGAGGGACCACTATAAACATCAAACGAACTGTGGCTGCACCAT5650.11641755370661129No Hit
ATTTCACTCTCACCATCAGCAATTTGCAGCCTGAGGATTGTGGAACCTACTACTGTCAGCAGAGCCACACTTCCC5580.11497521233325503No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT5560.11456311479801039No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5490.11312077342465415No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC5310.10941189560745236No Hit
CCTTCGGCGGAGGGACCACTATAAACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG5300.10920584683983005No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT5250.10817560300171844No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5250.10817560300171844No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC5230.1077635054664738No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5220.10755745669885149No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5220.10755745669885149No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGGGCC5160.10632116409311756No Hit
TGATAGTAGTGGTCAGCCCGATTACTACGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGC5120.10549696902262828No Hit
CCCACGTACGTGTTGATACCAATTCAAATAGACGCCAATGTTCTGACTTGCCCGGCAAGTGATGGTGACT5070.10446672518451666No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC5060.10426067641689436No Hit
GTTTTGGGGCTCTCCCACGTACGTGTTGATACCAATTCAAATAGACGCCA5050.10405462764927202No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGGGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC5010.10323043257878274No Hit
CTACTATCATATAGAAACTTCCTCCTCCTTGCACAGTAATAGACAGCCGT4970.10240623750829346No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG4960.10220018874067115No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGGGCC4930.10158204243780418No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT4880.10055179859969259No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG4880.10055179859969259No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)