FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485322
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15490.319169541046975No Hit
GTAATAGACAGCCGTGTCCTCGCCTCTCAGGCTGGTCATTTGCAGAAACAGCGAATTCTGGGCGTTGTCTCTGG13120.27033598312048496No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT12360.2546762767811886No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11460.2361318876951797No Hit
GTCTATTACTGTGCAAGGAGGAGGAAGTTTCTATATGATAGTAGTGGTCAGCCCGATTACTACGCTATGGACGT11120.22912622959602078No Hit
CTATAAACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG9930.2046064262489646No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA9820.20233988980511908No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9520.19615842677644943No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8960.18461969578959947No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8880.1829713056486209No Hit
CATCTATTCTGTTCGGAATTTGTTGAGAGGCGTCCCACCAAGGTTCAGTGGCGGCGGCTCTGGGACTTATTTC8590.1769958913875736No Hit
GTAGTAGGTTCCACAATCCTCAGGCTGCAAATTGCTGATGGTGAGAGTGAAATAAGTCCCAGAGCCGCCGCC8270.17040233082365935No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT8190.16875394068268076No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG8110.1671055505417022No Hit
GAATAGATGAGGAGTTTTGGGGCTCTCCCACGTACGTGTTGATACCAATT8080.16648740423883523No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7990.16463296533023436No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC7630.15721520969583083No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA7340.1512397954347835No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGGCGT7210.14856116145569334No Hit
AGTCAGAACATTGGCGTCTATTTGAATTGGTATCAACACGTACGTGGGAGAGCCCCAAAACTCCTCATCTATT7170.14773696638520406No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG7110.14650067377947013No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7100.1462946250118478No Hit
GTGTGGCTCTGCTGACAGTAGTAGGTTCCACAATCCTCAGGCTGCAAATTGCTGATGGTGAGAGTGAAATAAGTC6980.14382203980037994No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGGCGTCTATTTGAATTGGTATCAACACGT6930.14279179596226835No Hit
CTGCTACTCTGGCTCCGAGGGGCCAGATGTGACATCCAGATGACCCAGTC6890.14196760089177907No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6850.14114340582128979No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC6820.14052525951842282No Hit
ACCTACTACTGTCAGCAGAGCCACACTTCCCCGCTCACCTTCGGCGGAGGGACCACTATAAACATCAAACGA6810.1403192107508005No Hit
GTGCAGCCACAGTTCGTTTGATGTTTATAGTGGTCCCTCCGCCGAAGGTGAGCGGGGAAGTGTGGCTCTGCTGAC6460.13310750388401926No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGGGCCAGATGTGACATCC6410.13207726004590767No Hit
ATGTTTATAGTGGTCCCTCCGCCGAAGGTGAGCGGGGAAGTGTGGCTCTGCTGACAGTAGTAGGTTCCACAATC6410.13207726004590767No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6380.1314591137430407No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT6330.13042886990492908No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG6200.12775023592583892No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGGGCCAGATGTGACAT6200.12775023592583892No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6120.12610184578486036No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6110.12589579701723805No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC5990.12342321180577019No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG5860.12074457782668001No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT5770.11889013891807913No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5770.11889013891807913No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC5750.11847804138283449No Hit
CAGTAGTAGGTTCCACAATCCTCAGGCTGCAAATTGCTGATGGTGAGAGTGAAATAAGTCCCAGAGCCGCCGCC5720.11785989507996752No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG5560.11456311479801039No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC5500.11332682219227647No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTTTATAGTGGTCC5440.11209052958654254No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC5430.11188448081892023No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5270.10858770053696308No Hit
CTACTACTGTCAGCAGAGCCACACTTCCCCGCTCACCTTCGGCGGAGGGA5240.10796955423409613No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG5240.10796955423409613No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC5170.10652721286073988No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG5130.1057030177902506No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA5090.1048788227197613No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5020.10343648134640507No Hit
GAGCCACACTTCCCCGCTCACCTTCGGCGGAGGGACCACTATAAACATCAAACGAACTGTGGCTGCACCAT4980.10261228627591579No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGGGCC4920.10137599367018187No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG4910.10116994490255954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)