FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713635
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTATACGGCCGCGTCATCGCCTCTCAGGCTGTTCATCTGTAGATACACCGTATTCTTGGAATTGTCTCTGG42420.5944215180029007No Hit
GTATACTACTGTGCGAAAAGTCCCTTGCATTACTATGATAGTACTGGTTATTATGCCTACTTTGACGACTGGGGC25530.3577459065208405No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTTC21230.29749101431404007No Hit
ACCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCCTCTCCAGAGACAATTCCAAGAATACGGTGTATCT18190.25489220680039515No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17590.24648454742270207No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA17000.23821701570130388No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCACCTGCTTCTTTTATATGAGCTGCAGTAATAATC16900.23681573913835502No Hit
CTCCTGCACTGGGACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAGCAGAATTCAGGCAAAGC16290.22826795210436707No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16000.2242042500718154No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15120.21187301631786556No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14440.20234433568981341No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14410.20192395272092878No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGGACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGT14380.20150356975204411No Hit
CTCCTGTGCAGCCTCTGGATTCACCATTAGCACCTTTGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG14210.19912139959503108No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14150.19828063365726176No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14140.1981405060009669No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13710.19211501678028683No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC13400.1877710594351454No Hit
GTAGATACACCGTATTCTTGGAATTGTCTCTGGAGAGGGTGAACCGGCCC13250.18566914459072215No Hit
GTATGTGGTAGCACCACTACCACTGATACCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCT12630.1769812299004393No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12540.17572008099378533No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC12270.17193663427382344No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT12130.16997484708569507No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11990.16801305989756668No Hit
CTCCAGAGACAATTCCAAGAATACGGTGTATCTACAGATGAACAGCCTGAGAGGCGATGACGCGGCCGTATACT11750.16464999614648945No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11320.1586245069258094No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11170.15652259208138616No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGC11080.15526144317473217No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT11030.15456080489325777No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA10790.15119774114218054No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG10790.15119774114218054No Hit
GTATACGGCCGCGTCATCGCCTCTCAGGCTGTTCATCTGTAGATACACCG10690.14979646457923168No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG10680.1496563369229368No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATC10620.14881557098516748No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC10300.1443314859837312No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10200.14293020942078233No Hit
GATTATTACTGCAGCTCATATAAAAGAAGCAGGTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTC10160.1423696987956028No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10100.14152893285783347No Hit
GCCGTATACTACTGTGCGAAAAGTCCCTTGCATTACTATGATAGTACTGG10060.14096842223265396No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10030.1405480392637693No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9980.13984740098229487No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCAGGCCCCAGTCGTCAAAGTAGGCATAAT9940.13928689035711533No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG9900.1387263797319358No Hit
GTCGATCACCATCTCCTGCACTGGGACCAGCAGTGACGTTGGTGGTTATA9850.13802574145046137No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9840.1378856137941665No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC9810.13746523082528184No Hit
CCATTAGCACCTTTGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG9810.13746523082528184No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9720.13620408191862787No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTTCAGTGTGAGGTGCAGCTGTTGGAGT9680.13564357129344834No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9520.13340152879273018No Hit
GAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAAC9400.13171999691719158No Hit
GTACTATCATAGTAATGCAAGGGACTTTTCGCACAGTAGTATACGGCCGCGTCATCGCCTCTCAGGCTGTTCAT9370.13129961394830691No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG9310.1304588480105376No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCATTAGCACCTTTGCC9300.13031872035424272No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9210.12905757144758875No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT9200.12891744379129386No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG9180.12863718847870412No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC8950.12541425238392173No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC8940.12527412472762686No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT8900.12471361410244734No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8770.12289195457061382No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAG8740.12247157160172918No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT8680.12163080566395988No Hit
CTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGAGAACTCACC8660.12135055035137009No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8590.1203696567573059No Hit
GGGTTCCCAGGCCCCAGTCGTCAAAGTAGGCATAATAACCAGTACTATCATAGTAATGCAAGGGACTTTTCGCAC8500.11910850785065194No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8490.11896838019435706No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCACCTGCTTCTTTTATATGAGCTGCAGTAATAATCAGC8420.11798748660029287No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCAGG8410.11784735894399799No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC8280.11602569941216448No Hit
GCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTG8280.11602569941216448No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCAAAGGTGCTAATGGTGAATCCAGAGGCTGCAC8260.11574544409957471No Hit
CTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGAGAACTC8220.11518493347439517No Hit
TCTGTAGATACACCGTATTCTTGGAATTGTCTCTGGAGAGGGTGAACCGG8200.11490467816180541No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8060.112942890973677No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC7960.11154161441072818No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT7790.10915944425371514No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7770.10887918894112536No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG7670.10747791237817651No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7610.1066371464404072No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC7480.10481548690857372No Hit
GTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCC7450.10439510393968905No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7430.10411484862709929No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG7340.10285369972044532No Hit
GGCTTGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCATTAGCACCTTTGCCATG7280.10201293378267601No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG7270.10187280612638112No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG7260.10173267847008625No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGC7170.10047152956343228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)