FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713635
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTATACGGCCGCGTCATCGCCTCTCAGGCTGTTCATCTGTAGATACACCGTATTCTTGGAATTGTCTCTGG37860.5305233067324332No Hit
GTATACTACTGTGCGAAAAGTCCCTTGCATTACTATGATAGTACTGGTTATTATGCCTACTTTGACGACTGGGGC23280.32621718385449144No Hit
ACCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCCTCTCCAGAGACAATTCCAAGAATACGGTGTATCT18850.2641406321158576No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTTC16610.23275203710580336No Hit
CTCCTGCACTGGGACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAGCAGAATTCAGGCAAAGC16400.22980935632361082No Hit
CTCCTGTGCAGCCTCTGGATTCACCATTAGCACCTTTGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG14960.20963097381714743No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCACCTGCTTCTTTTATATGAGCTGCAGTAATAATC14690.20584752709718554No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA14490.20304497397128785No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGGACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGT14350.20108318678315945No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14270.19996216553280038No Hit
CTCCAGAGACAATTCCAAGAATACGGTGTATCTACAGATGAACAGCCTGAGAGGCGATGACGCGGCCGTATACT14210.19912139959503108No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC13040.18272646380852955No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12680.17768186818191373No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12620.17684110224414443No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12600.17656084693155463No Hit
GTAGATACACCGTATTCTTGGAATTGTCTCTGGAGAGGGTGAACCGGCCC12500.1751595703686058No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12450.1744589320871314No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12410.17389842146195184No Hit
GTATGTGGTAGCACCACTACCACTGATACCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCT12350.17305765552418254No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12080.16927420880422064No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCAAAGGTGCTAATGGTGAATCCAGAGGCTGCAC11780.16507037911537412No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11380.1594652728635787No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG11210.15708310270656567No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10730.15035697520441121No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT10700.14993659223552655No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA10640.14909582629775725No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10620.14881557098516748No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT10460.14657352848444935No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10400.14573276254668002No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC10360.1451722519215005No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGC10320.14461174129632096No Hit
GATTATTACTGCAGCTCATATAAAAGAAGCAGGTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTC10030.1405480392637693No Hit
GTGTATCTACAGATGAACAGCCTGAGAGGCGATGACGCGGCCGTATACTACTGTGCGAAAAGTCCCTTGCATT9990.13998752863858974No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG9960.1395671456697051No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCAGGCCCCAGTCGTCAAAGTAGGCATAAT9900.1387263797319358No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC9880.138446124419346No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC9800.13732510316898694No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG9710.13606395426233298No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG9680.13564357129344834No Hit
GTATACGGCCGCGTCATCGCCTCTCAGGCTGTTCATCTGTAGATACACCG9670.13550344363715344No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT9190.128777316134999No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC9080.12723591191575526No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCATTAGCACCTTTGCC9040.12667540129057572No Hit
CCATTAGCACCTTTGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG8860.1241531034772678No Hit
GCCGTATACTACTGTGCGAAAAGTCCCTTGCATTACTATGATAGTACTGG8860.1241531034772678No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8790.12317220988320361No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG8760.12275182691431895No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8670.12149067800766497No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8650.12121042269507522No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8510.11924863550694684No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG8420.11798748660029287No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATC8410.11784735894399799No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC8350.11700659300622868No Hit
GTCGATCACCATCTCCTGCACTGGGACCAGCAGTGACGTTGGTGGTTATA8200.11490467816180541No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG8180.11462442284921565No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAA8030.11252250800479237No Hit
GCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTG7950.11140148675443327No Hit
GTACTATCATAGTAATGCAAGGGACTTTTCGCACAGTAGTATACGGCCGCGTCATCGCCTCTCAGGCTGTTCAT7940.1112613590981384No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTTCAGTGTGAGGTGCAGCTGTTGGAGT7940.1112613590981384No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7860.11014033784777932No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG7850.11000021019148445No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC7710.10803842300335606No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7680.10761804003447141No Hit
TCTGTAGATACACCGTATTCTTGGAATTGTCTCTGGAGAGGGTGAACCGG7480.10481548690857372No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7400.10369446565821465No Hit
GAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAAC7390.10355433800191974No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7170.10047152956343228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)