FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1521258
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63300.416102988447719No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT61950.4072287540969382No Hit
ACTTAATAGTTACCCTCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT51680.3397188379617395No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45180.29699104293946194No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG43140.28358108880939326No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG42950.28233212249335743No Hit
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGACACAGCGTGTTCTTGGAATTGTCTCTGG42530.2795712495842257No Hit
CTATTAAGTAGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC41150.2704998100256498No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCC41070.2699739294715295No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39620.2604423444280983No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG38290.25169958021584765No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTC37410.24591489412052392No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36300.23861830143210422No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT36180.2378294806009237No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAA34950.22974406708132347No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT34060.2238936459167347No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC33780.2220530639773135No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA33180.21810895982141096No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA32350.21265294907241244No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC32300.21232427372608723No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAGAGGGTAACTATTAAGTAGTTGAC30190.19845417411116328No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT29750.1955618310635014No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCC29570.19437859981673064No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG28830.1895142046911175No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28140.18497848491182955No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA27750.18241481721049288No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25680.1688076578726291No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT24630.16190547559979965No Hit
GTGTATTACTGTGCGAAAGATAGGTCGGACTACTGTAGTGGTGGTAGCTG23900.15710681554345154No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23810.15651519992006616No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT23320.15329418152607907No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23000.1511906593095977No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22900.15053330861694728No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCC22690.14915287216238138No Hit
ATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTC22530.14810111105414073No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC22440.14750949543075534No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAT22410.14731229022296022No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC22130.145471708283539No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC21580.14185627947396168No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC21230.1395555520496852No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20890.13732055969467374No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGG20740.13633453365569811No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACTACTTAATAGTTAC20690.1360058583093729No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTG20490.13469115692407205No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTT20390.13403380623142164No Hit
GGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATC19730.12969529165992882No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19660.12923514617507353No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC19630.1290379409672784No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT19520.12831485520536293No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT19040.1251595718806409No Hit
ACTTATTACTGTCAACTACTTAATAGTTACCCTCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGA18970.1246994263957856No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18760.1233189899412197No Hit
AATCAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTGTCTG18700.12292457952562945No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT18320.12042664689355784No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAGCTACTGAAGGTGAATCCAGAGGCTGCAC18090.11891474030046185No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17920.11779724412295614No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG17550.11536504656014955No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC17520.11516784135235443No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG17400.11437902052117394No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC17040.1120125580276324No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16700.10977756567262095No Hit
ATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAACTGCTAATGCC16620.10925168511850061No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTG16520.10859433442585019No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16480.10833139414879No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG16330.10734536810981439No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16240.106753752486429No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16220.10662228234789892No Hit
GTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTG16180.10635934207083875No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16150.10616213686304361No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT16090.10576772644745336No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTG15980.10504464068553789No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCC15980.10504464068553789No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15930.10471596533921268No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15470.10169215215302073No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)