FastQCFastQC Report
Wed 8 May 2024
EGAF00004717965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069434
Total Bases78.3 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT55570.5196206591524114No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48860.45687718924215986No Hit
GAGTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGTCC48350.4521083114993539No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36840.3444812863626928No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC36820.3442942715492494No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34430.32194600134276635No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT33810.3161485421260218No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32890.30754586070762663No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG31860.29791459781529295No Hit
CCTTCCACCCTGTCTGCATCTATAGGAGACAGAGTCACCATCATCTGCCG30490.2851040830944219No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28640.26780521285090997No Hit
GTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGTCCCT27650.25854797958546294No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27530.2574258907048027No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27110.2534985796224919No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26000.24311925747638471No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCCCCTGGGCAAAGGTG25960.24274522784949798No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA25660.23994000564784737No Hit
GGGCAAAGGTGACGACCCCCGGAGAATAAGTATCAGAGTGCTGGCAGTAA25360.23713478344619676No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG25330.2368542612260317No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25110.23479709827815462No Hit
CCTTTGCCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCA24680.2307762797891221No Hit
CTCTGATACTTATTCTCCGGGGGTCGTCACCTTTGCCCAGGGGACCAACC24640.23040225016223537No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT24120.22553986501270767No Hit
GTATCAGAGTGCTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCT23710.22170606133711854No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC23580.22049046504973657No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA23220.2171241984077559No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCATCTGCCGGGCCAGTCAGA22050.20618383182131858No Hit
ATAGGAGACAGAGTCACCATCATCTGCCGGGCCAGTCAGAGTATTAGTAG22040.20609032441459688No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA21050.19683309114914993No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCC20760.19412137635422103No Hit
TCACTAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGC20710.1936538393206126No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20330.1901005578651885No Hit
CTAATACTCTGACTGGCCCGGCAGATGATGGTGACT20220.18907197639124995No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC20180.18869794676436322No Hit
GGATATGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGA20170.1886044393576415No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20030.1872953356635379No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19770.18486414308877408No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA19110.17869265424514277No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18860.1763549690771006No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT18670.17457832834938855No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG18310.17121206170740783No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCC17810.16653669137132351No Hit
GTCTCCTTCCACCCTGTCTGCATCTATAGGAGACAGAGTCACCATCATCT17550.16410549879655967No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17240.16120676918818738No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC17170.1605522173411356No Hit
CCTTAGTGATCAGGAGCTTAGGGACTTTCCCTGGTTTCTGCTGATACCAG17110.15999117290080547No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16990.15886908402014524No Hit
CTCCAGGTTGGTCCCCTGGGCAAAGGTGACGACCCCCGGAGAATAAGTAT16430.15363266924373079No Hit
GGTATCAGCAGAAACCAGGGAAAGTCCCTAAGCTCCTGATCACTAAGGCG16360.152978117396679No Hit
GTCCCTAAGCTCCTGATCACTAAGGCGTCTAGTTTAGAAAGTGGGGTCCC16290.15232356554962717No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16120.15073393963535853No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15990.1495183433479766No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAGC15930.14895729890764647No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC15690.146713121146326No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15660.14643259892616092No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTAGTGATCAGGAGC14910.13941954342203444No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14910.13941954342203444No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG14790.13829745454137424No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14640.13689484344054892No Hit
AACCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCC14570.13624029159349713No Hit
ATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGTCCCTAA14400.13465066567922845No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14070.1315649212574128No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC13830.12932074349609232No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT13790.1289467138692056No Hit
CTATAGGAGACAGAGTCACCATCATCTGCCGGGCCAGTCAGAGTATTAGT13570.12688955092132848No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13530.12651552129444174No Hit
CATCAAGGTTCAGCGGCAGTGGATATGGGACAGAATTCACTCTCACCATC13340.12473888056672969No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13250.12389731390623451No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13150.12296223983901763No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13080.12230768799196584No Hit
AGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATATGGGAC13060.12212067317852247No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13050.12202716577180078No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC13010.12165313614491405No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC12940.12099858429786223No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA12810.11978298801048032No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12720.11894142134998512No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12360.11557515470800442No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12110.11323746953996226No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT12050.11267642509963213No Hit
ACTTATTACTGCCAGCACTCTGATACTTATTCTCCGGGGGTCGTCACCTT11940.11164784362569359No Hit
TTCTAAACTAGACGCCTTAGTGATCAGGAGCTTAGGGACTTTCCCTGGTT11770.11005821771142492No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11750.10987120289798155No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11740.10977769549125986No Hit
ATATGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATT11710.1094971732710948No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11710.1094971732710948No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11650.10893612883076469No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11560.1080945621702695No Hit
GCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTT11510.10762702513666107No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11330.10594389181567071No Hit
TACTTATTCTCCGGGGGTCGTCACCTTTGCCCAGGGGACCAACCTGGAGA11320.10585038440894903No Hit
GCAGTGGATATGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCT11290.10556986218878397No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC11250.10519583256189723No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11240.10510232515517555No Hit
GTATCAACGCAGAGTACGGGAGGAGTCAGACCCAGTCAGGACACAGCATG10980.1026711325804117No Hit
CCTACTAATACTCTGACTGGCCCGGCAGATGATGGTGACT10960.10248411776696834No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10950.10239061036024664No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10930.10220359554680325No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10900.10192307332663819No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10840.10136202888630808No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)