FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326258
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACATTAAATTGTTGGTGCCTGCATATGAGCTGCAGTAATAAT51380.38740576871166843No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG45420.34246730274200043No Hit
CTCCTGGACAGTCAGTCGCCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATGGCTGGGTCTCCTGGT43050.3245974765090955No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACATTAAATTGTTGGTGCCTGCATATGAGCTG40900.30838645271131254No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC39670.299112239096767No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCCTGATTTATGAGGTCA39180.2954176336730862No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCGCCATCTCCTG37870.28554021917304173No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34850.2627693857454583No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATGGCTGGGTCTCCTGGTACCAACACCACCCAGGCAAAGC31680.23886755065756438No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA30780.23208154069570175No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC30490.22989493748576825No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28650.21602131711929354No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG28320.21353311346661058No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC28250.21300531269179904No Hit
GTGTTGGTACCAGGAGACCCAGCCATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGCGACTG26510.19988569343219795No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC26490.19973489321082324No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT24140.18201586719929305No Hit
GATTATTACTGCAGCTCATATGCAGGCACCAACAATTTAATGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGT23590.1778688611114881No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG23570.17771806089011338No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACATTAAATTGTTGGTGCCTGCATATGAGCTGCAGTAATAATC23220.1750790570160557No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22490.16957484893587824No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCGC22380.16874544771831723No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22380.16874544771831723No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC22040.16618184395494692No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC21750.1639952407450134No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT21190.1597728345465211No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21180.15969743443583376No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA20630.15555042834802882No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGGTCCATGTAGAATGTGCTCGTGGACGTGTCCCTGGT20430.15404242613428157No Hit
TTTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT20370.15359002547015738No Hit
CCCCTGAGGGCCGCTTAATGACCTCATAAATCAGGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGG19710.14861361816479146No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATTTCTGGGCTCC19460.1467286153976074No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19130.14424041174492444No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG19100.14401421141286236No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA18960.1429586098632393No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18930.1427324095311772No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT18860.14220460875636565No Hit
GTCTGGGGCTGAGGTGAAGGAGCCTGGGACCTCAGTAAAGGTTTCCTGCA18820.14190300831361619No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC18460.13918860432887115No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18380.13858540344337225No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGT18340.13828380300062282No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAAATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAG18150.13685120089756292No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC17940.13526779857312832No Hit
GTCATTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTC17850.13458919757694204No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC17620.1328549950311327No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC17280.13029139126776237No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17190.1296127902715761No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCGCCATCTCCTGCACTGGAACC16890.12735078695095522No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC16840.12697378639751844No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16590.12508878363033438No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG16500.1244101826341481No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATTTCTGGGCTCCAGG16160.12184657887077778No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15930.12011237632496845No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15890.11981077588221899No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC15800.11913217488603273No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15460.11656857112266242No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCGC15330.11558836968372671No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT15300.11536216935166461No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15290.11528676924097725No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGAC15290.11528676924097725No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15230.11483436857685307No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA15100.11385416713791736No Hit
GTTTGGTCCCTCCGCCGAACATTAAATTGTTGGTGCCTGCATATGAGCTGCAGTAATAATCAGCCTCATCCTC14640.11038576204629867No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT14540.10963176093942506No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCGCCATCT14450.1089531599432388No Hit
CATATGCAGGCACCAACAATTTAATGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCC14030.10578635529436957No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTC13310.10035754732487948No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)