FastQCFastQC Report
Wed 8 May 2024
EGAF00004717971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107174
Total Bases81 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT57380.5182563896912319No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50980.46045156407213317No Hit
GTACAGTAGCTGGCCTCGGACGTTCGGCCAAGGGACCAGGGTGGAAATCA46060.4160141043774511No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC43860.3961436955708859No Hit
CTACTGTACTGCTGACAGTAATAAACCGCAAAATCTTCAGACTGCAGGCT40440.36525424188068No Hit
GTATTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCC37930.3425839118331897No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35960.3247908639473109No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34550.31205573830310324No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT33400.3016689336996714No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT33390.3015786136596416No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32190.29074020885606056No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG32080.2897466884157323No Hit
CTCCTGCAGGGCCAGTCACAGTATTAGCAGCAACTTAGCCTGGTACCAGC31540.2848694062541208No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAGGAGCCACCCTCTCCT31240.28215980505322563No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29460.26608283792791376No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28170.2544315527640642No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCAGGT28030.25316707220364637No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27600.24928331048236324No Hit
GTCTGTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCACA25350.2289613014756488No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCGGTTTA25140.22706458063502213No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24600.2221872984734107No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24350.21992929747266465No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC24050.21721969627176937No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA24040.21712937623173953No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC23910.21595521571135162No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA23330.21071665338962078No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC22310.20150400930657691No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT21010.18976240410269749No Hit
CAGTATTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTC20420.18443352174093683No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC20190.18235616082025047No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC20050.1810916802598327No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18890.17061455561637104No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT18570.16772431433541612No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA18240.16474375301443134No Hit
TCCATGATGCATCCACCTGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGC18210.16447279289434183No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17840.16113095141323766No Hit
GTACTGCTGACAGTAATAAACCGCAAAATCTTCAGACTGCAGGCTGCTGA17820.160950311333178No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17590.1588729504124916No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC17570.15869231033243195No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC17360.15679558949180528No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17220.1555311089313875No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG16930.15291182777052206No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCA16780.15155702717007447No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT16710.15092478688986555No Hit
CCAGTGGCCCAGGTGGATGCATCATGGATGACGAGCCTGGGAGCCTGGCC16130.1456862245681347No Hit
CTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT16070.14514430432795566No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16060.14505398428792585No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC15990.14442174400771693No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG15730.14207342296694106No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15720.14198310292691121No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15640.14126054260667248No Hit
CAGCAGTACAGTAGCTGGCCTCGGACGTTCGGCCAAGGGACCAGGGTGGA15460.13963478188613535No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14900.1345768596444642No Hit
GGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGT14700.13277045884386734No Hit
GTTTATTACTGTCAGCAGTACAGTAGCTGGCCTCGGACGTTCGGCCAAGG14440.13042213780309148No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCGTCATCCATGATGCATCCACCT14260.12879637708255431No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG14020.12662869612183814No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCC14000.12644805604177844No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13950.12599645584162922No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13870.12527389552139048No Hit
GTAATAAACCGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGA13800.12464165524118162No Hit
CCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGT13510.1220223740803162No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC13490.1218417340002565No Hit
ACCCTCTCCTGCAGGGCCAGTCACAGTATTAGCAGCAACTTAGCCTGGTA13140.11868053259921205No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13140.11868053259921205No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT13060.11795797227897331No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACT13060.11795797227897331No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13040.11777733219891363No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13040.11777733219891363No Hit
TCCGAGGCCAGCTACTGTACTGCTGACAGTAATAAACCGCAAAATCTTCA12930.11678381175858536No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCC12860.11615157147837649No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12830.11588061135828695No Hit
CTCCCAGGCTCGTCATCCATGATGCATCCACCTGGGCCACTGGTGTCCCA12730.11497741095798854No Hit
CACCTGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGA12640.11416453059771997No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12550.11335165023745139No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12550.11335165023745139No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA12540.11326133019742153No Hit
GCTAATACTGTGACTGGCCCTGCAGGAGAGGGTGGCTCCTTCCCCTGGAG12530.1131710101573917No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12490.11280972999727233No Hit
CTGCAGGAGAGGGTGGCTCCTTCCCCTGGAGACACAGACAGGGTGGCTGG12370.11172588951691423No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12320.11127428931676502No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12270.11082268911661582No Hit
GTCACAGTATTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAG12200.11019044883640691No Hit
GAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCT12190.11010012879637708No Hit
CATCATGGATGACGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAG12140.10964852859622787No Hit
ATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA11960.10802276787569072No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11820.10675828731527294No Hit
CAGTAATAAACCGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGT11650.10522284663476564No Hit
CACCATCAGCAGCCTGCAGTCTGAAGATTTTGCGGTTTATTACTGTCAGC11460.10350676587419863No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11360.10260356547390022No Hit
GGTTTCTGCTGGTACCAGGCTAAGTTGCTGCTAATACTGTGACTGGCCCT11360.10260356547390022No Hit
CTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCACAGTATTAGC11360.10260356547390022No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT11260.1017003650736018No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11230.10142940495351227No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11220.10133908491348244No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCACAGTATTAGCAGCAACTTAGCC11210.1012487648734526No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11170.10088748471333324No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11150.10070684463327353No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAG11110.10034556447315417No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11090.10016492439309449No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)