FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025299
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGAAGACTGTGTTCGTGGATGTGTCTGTGGT31920.31132381871044446No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30370.2962062773883521No Hit
GTCTATTACTGTGCGAGAGATGGTAGCTGGGCTACGGTGACCCCACTAAACTGGGTCGACCCCTGGGGCCAGGGG23840.23251753878624676No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT23580.22998169314512157No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC21290.20764674499828834No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATT18630.1817030934390846No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18550.1809228332418153No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18280.1782894550760315No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16850.164342304049843No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16570.16161139335940053No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCGGCAGCTACTTAGCCTGGTACC16500.1609286656867899No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG16130.15731996227441947No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGG16120.1572224297497608No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15950.15556437683056357No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA15730.15341866128807305No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT15490.15107788069626518No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15150.14776177485787073No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15050.14678644961128412No Hit
CATCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTC15010.14639631951264948No Hit
GTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTTAAGGTCTCCTGCAAGGCCTCTGGTTACACCTTTACT14920.14551852679072155No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGTGAGCTGCCATATTGCTGAC14660.14298268114959636No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14580.14220242095232707No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14480.14122709570574046No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14310.13956904278654325No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14190.13839865249063932No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGGC13980.13635046947280746No Hit
CTATTACTGTCAGCAATATGGCAGCTCACCTCGGACGTTCGGCCAAGGGA13300.12971825779601853No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG13290.12962072527135987No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT13260.1293281276973839No Hit
TCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT13190.12864540002477326No Hit
CTCCCAGGTTCAGCTGGTGCAGTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTTAAGGTCTCCTGCAAGGC13140.12815773740147995No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12960.12640215195762408No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12850.12532929418637878No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGGAGCATCCTTTTCTTGGTGGCAGC12560.12250085097127764No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCCGCTAACACTCTG12290.11986747280549383No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCACTTACAATGGTATTACAGACTATGCACAGAAATTTC12260.11957487523151782No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12250.11947734270685917No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC12110.11811188736163793No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG11810.1151859116218781No Hit
GTATTACAGACTATGCACAGAAATTTCAGGGCAGAGTCACCATGACCACA11800.11508837909721946No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC11590.11304019607938758No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11310.11030928538894509No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG11250.10972409024099311No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGTG11140.10865123246974785No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC11060.10787097227247856No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT11040.10767590722316124No Hit
CAGTAATAGATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC10930.10660304945191597No Hit
GACCTGGAGCATCCTTTTCTTGGTGGCAGCAGCAACAGGTGCCCACTCCCAGGTTCAGCTGGTGCAGTCTGG10800.10533512663135339No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10690.10426226886010813No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10560.10299434603954555No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA10530.10270174846556956No Hit
GTATCAACGCAGAGTACGGGACCCAAAAACCACACCCCTCCTTGGGAGAA10370.101141228071031No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC10260.10006837029978573No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10260.10006837029978573No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)