FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004717997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645799
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT81840.4972660695504129No Hit
CTCCTAAGCTGCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCT81280.49386346692396826No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT72790.44227758067661965No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTCGTTCCTATTCTTGAAGCTGTATGAAACACT65630.39877287566707725No Hit
ATATTATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT61550.3739824851029804No Hit
GTTTCATACAGCTTCAAGAATAGGAACGACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTC59220.3598252277465231No Hit
ATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA53310.32391561788529466No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC50110.3044721743056108No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG47550.2889174194418638No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG47320.287519921934574No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT46450.28223373571134747No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG42580.25871932113216745No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTCAT41320.2510634652226669No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA40210.24431902073096412No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA39500.24000500668672178No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCATCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTC37480.22773133292704636No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGACTTCACTCTCAC37210.22609079237501054No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC35570.21612602754042262No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG35330.21466776927194633No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC34920.21217657806329934No Hit
TTATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT33340.2025763777958305No Hit
GTATGAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGG32920.20002442582599697No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT32850.19959910049769136No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCTTCTACCCGGGAA32800.19929529669175883No Hit
GAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCATCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAGT32650.19838388527396114No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTCATACAGCTTCAAGAATAGGAACG31080.18884444576767878No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTACTATAATATTGCTGAC30790.18708238369326996No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC30610.18598868999191273No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA30470.1851380393353016No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGGCTGATGATG29880.18155315442529738No Hit
GTCAGGGACCCCGGATTCCCGGGTAGAAGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGT29760.18082402529105923No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29470.17906196321665038No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT29410.17869739864953132No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTCATACAGCTTCA29070.17663153276918994No Hit
TCTGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC28630.1739580592769834No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28580.17365425547105084No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA28290.171892193396642No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27530.1672743755464671No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC26760.16259579693510567No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGGCTGATGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACT26710.1622919931291731No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25620.15566907015984333No Hit
GGTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGGCTGATGATGTGGCAGTTTATTACTGTCAGCAAT25510.1550007017867917No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCATCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAGT25320.15384624732424798No Hit
GTAATACATGGCCGTGTCGTCAGATCTCAGCCTGCTCAATTCCATGAAGGCTGTATTGTTGGACGTGTCCCTGGT25130.15269179286170426No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25030.15208418524983913No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC24910.151355056115601No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT24360.14801321425034283No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTCATACAGCTTCAAGA24180.14691952054898563No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC24020.14594734837000145No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC22970.13956746844541768No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22900.1391421431171121No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC22780.13841301398287398No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22710.13798768865456837No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA22440.13634714810253257No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTCGTTCCTATTCTTGAAGCTGTATGAAACACTCTGGCTGGACTTGC21660.13160780872998465No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT21330.12960270361082976No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATT21180.12869129219303208No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC21020.1277191200140479No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACAGTTTCACCGG20820.12650390479031765No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20760.1261393402231986No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT20320.12346586673099207No Hit
ATGTATTACTGTGCGAGAGTCAGAGGGGGGGATGACAACAACCCACGGGC20270.1231620629250595No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC20190.1226759768355674No Hit
CACCATCAGCAGCCTGCAGGCTGATGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCGCTCACTTT20010.1215822831342102No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA19870.12073163247759904No Hit
CCTCTGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCA19850.120610110955226No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19700.11969869953742833No Hit
CTCTGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG19480.11836196279132506No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGG19220.11678218300047576No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18900.11483783864250738No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTGTTTCATACAGCTTCAAGAATAGGAACGACTTAGCTTGGTACCAGC18050.10967317394165388No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA18010.10943013089690781No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTCATAC17660.10730350425537992No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGAAGCCCAGTAAAT17250.10481231304673291No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17220.10463003076317338No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTCTGGGGACATCGTGATG17070.10371861934537571No Hit
GGATCTCTGGTGCCTCTGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG17050.10359709782300269No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT17020.10341481553944316No Hit
GGCTGATGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCGC17010.10335405477825664No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16790.10201731803215339No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC16770.10189579650978035No Hit
CTACATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATGAATCCTAACAGTGGTGG16750.10177427498740733No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16710.10153123194266128No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16570.10068058128605013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)