FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004717998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645799
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT81940.49787367716227804No Hit
CTCCTAAGCTGCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCT65890.4003526554579265No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT65100.3955525553241921No Hit
ATATTATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT59770.36316706961178125No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTCGTTCCTATTCTTGAAGCTGTATGAAACACT58390.35478208456804267No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCATCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTC52140.3168066088264727No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49600.30137337548509874No Hit
ATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA48780.29639099306780475No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT47470.2884313333523717No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGAAGCCCAGTAAATGAGCAGCTT45990.27943874069676794No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44550.2706891910859102No Hit
GTTTCATACAGCTTCAAGAATAGGAACGACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTC44150.2682587606384498No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG43360.26345866050471534No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA41410.25161031207334555No Hit
GAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCATCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAGT41130.2499090107601232No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA35910.21819189342076403No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTACTATAATATTGCTGAC34500.20962462609346583No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCATCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAGT34060.20695115260125932No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG33780.205249851288037No Hit
TTATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT32680.1985661675575207No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTCAT32520.19759399537853647No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC32390.19680410548311186No Hit
GTAATAAACTGCCACATCATCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCC31720.19273313448361556No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGACTTCACTCTCAC31650.19230780915530996No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31480.19127487621513928No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG31020.18847988120055972No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC30790.18708238369326996No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT30580.18580640770835322No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT29430.17881892017190434No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC28850.17529479602308665No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGGCTGATGATG28620.17389729851579688No Hit
GTAATACATGGCCGTGTCGTCAGATCTCAGCCTGCTCAATTCCATGAAGGCTGTATTGTTGGACGTGTCCCTGGT28340.17219599720257456No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27900.16952252371036802No Hit
GAGTGAAGTCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG27330.16605916032273685No Hit
GTCAGGGACCCCGGATTCCCGGGTAGAAGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGT26620.1617451462784945No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC26550.16131982095018893No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGGCTGATGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACT26470.16083373486069685No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA26310.15986156268171264No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA25980.15785645756255776No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC25730.15633743853289497No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC25320.15384624732424798No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT25280.15360320427950194No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25170.1529348359064503No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24800.15068668774254937No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCTTCTACCCGGGAA24640.1497145155635652No Hit
GGTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGGCTGATGATGTGGCAGTTTATTACTGTCAGCAAT24440.14849930033983494No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT24070.146251152175934No Hit
GTATGAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGG23720.14412452553440608No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23480.1426662672659298No Hit
TCTGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC22950.13944594692304468No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC22600.13731932028151678No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC22380.13598258353541348No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC22310.13555725820710793No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTCATACAGCTTCA22000.13367367461032603No Hit
TCATCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC21860.1328230239537149No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTCATACAGCTTCAAGAATAGGAACG21520.1307571580733735No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20220.12285825911912693No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTCGTTCCTATTCTTGAAGCTGTATGAAACACTCTGGCTGGACTTGC20100.12212912998488878No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19690.1196379387762418No Hit
CACCATCAGCAGCCTGCAGGCTGATGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCGCTCACTTT19430.1180581589853925No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19350.11757207289590041No Hit
ATGTATTACTGTGCGAGAGTCAGAGGGGGGGATGACAACAACCCACGGGC19020.11556696777674552No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC18600.11301501580691202No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACAGTTTCACCGG18570.11283273352335249No Hit
CAGTAATAAACTGCCACATCATCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCC18560.11277197276216597No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT18520.11252892971741993No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC18380.11167827906080877No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18090.10991621698639993No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17970.10918708785216179No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC17800.10815415491199107No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGT17790.10809339415080456No Hit
GGCTGATGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCGC17730.10772882958368549No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT17320.10523763837503851No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA17290.10505535609147898No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17070.10371861934537571No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16940.10292872944995106No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16620.10098438509198268No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTCATACAGCTTCAAGA16520.10037677748011756No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCATCAGC16490.10019449519655801No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG16460.10001221291299849No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)