FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143729
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC82580.7220241857992584No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCAAAAACCAT74380.6503288803553988No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC69200.605038431306717No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43760.3826081178321088No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA39190.34265110004205546No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC38160.3336454702119121No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC37210.325339306776343No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC35040.30636628082351675No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG34670.3031312487486109No Hit
ATTCAAGACACAGCGATACAAGAGACATGGTTTTTGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGG34420.3009454162655664No Hit
CCCCTGTGTTGGTCATCAGTGAGAACACCAAGCGACCCTCCGGCATCCCT34000.2972732176940517No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG32260.28205982361206194No Hit
GTGTTGGTCATCAGTGAGAACACCAAGCGACCCTCCGGCATCCCTGAGAGATTCTCTGGCTCCAGCTCGGGGACG31960.27943682463240854No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC31480.27524002626496313No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT29830.26081353187686945No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29480.25775336640060714No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG29350.256616733509424No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC28550.2496220695636816No Hit
GTCTTGAATAGCAATAGTAGTCAGCTTCATCCTCTACCTGGGCCCCAGCGATAGTCAAGGTGGCCATCGTCCCCG28530.24944720296503803No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG28370.24804827017588957No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC28040.24516297129827083No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCAAAAACCATGTCTCTTGTATCGCTGTGTCTTGAATAGCAATAGT28010.24490067140030553No Hit
ACACAGGGGCCTGGCCTGACTTCTGCTGATACCAATAAACATACCTCTTT27900.24393890510776592No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCAAAAACCATGTCTCTTGTATCGCTGTGTCTTGAATAGCAATAGT27760.242714838917261No Hit
GTGAGAACACCAAGCGACCCTCCGGCATCCCTGAGAGATTCTCTGGCTCCAGCTCGGGGACGATGGCCACCTTG27670.241927939223365No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG27140.23729397435931066No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26370.2305616103115336No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25980.22715171163798417No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGGCAAAGAGGTATGTTTATTGGTATCAGCAGA25130.2197198811956329No Hit
CTCTTGTATCGCTGTGTCTTGAATAGCAATAGTAGTCAGCTTCATCCTCT24410.21342468364446474No Hit
ATACAAGAGACATGGTTTTTGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTC24260.21211318415463803No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA23740.20756665258990548No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG23390.20450648711364317No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA23340.20406932061703426No Hit
GCTCTGGAGATGCATTGGCAAAGAGGTATGTTTATTGGTATCAGCAGAAG23330.2039818873177125No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG23300.20371958741974716No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22550.19716208997061369No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC22440.19620032367807408No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC22320.19515112408621274No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC21940.1918286587119851No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC21640.1892056597323317No Hit
ACCTGGGCCCCAGCGATAGTCAAGGTGGCCATCGTCCCCGAGCTGGAGCC21510.18806902684114854No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG21010.18369736187505956No Hit
GGCCAGGCCCCTGTGTTGGTCATCAGTGAGAACACCAAGCGACCCTCCGGCATCCCTGAGAGATTCTCTGGCTCC20250.1770524311266043No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20170.17635296473203005No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTC19760.17276819945983707No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC19730.17250589956187173No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19530.17075723357543615No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19080.16682273510595605No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC18880.16507406911952044No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18850.1648117692215551No Hit
GGATGAAGCTGACTACTATTGCTATTCAAGACACAGCGATACAAGAGACATGGTTTTTGGCGGAGGGACCA18810.16446203602426798No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18480.16157673714664925No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCAAAAACCATGT18040.15772967197649093No Hit
GGATCACCTGCTCTGGAGATGCATTGGCAAAGAGGTATGTTTATTGGTATCAGCAGAAGTCAGGCCAGGCCC17840.15598100599005532No Hit
GGCCCAGGTAGAGGATGAAGCTGACTACTATTGCTATTCAAGACACAGCGATACAAGAGACATGGTTTTTGGCGG17690.15466950650022865No Hit
GTGTTCTCACTGATGACCAACACAGGGGCCTGGCCTGACTTCTGCTGATA17600.15388260680633262No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC17170.1501229749354961No Hit
GAGGTATGTTTATTGGTATCAGCAGAAGTCAGGCCAGGCCCCTGTGTTGGTCATCAGTGAGAACACCAAGCGACC16820.1470628094592338No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA16780.14671307626194666No Hit
GGTAGAGGATGAAGCTGACTACTATTGCTATTCAAGACACAGCGATACAAGAGACATGGTTTTTGGCGGAGGGAC16710.1461010431666942No Hit
GACCAACACAGGGGCCTGGCCTGACTTCTGCTGATACCAATAAACATACCTCTTTGCCAATGCATCTCCAGAGC16330.14277857779246655No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTC16290.14242884459517946No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16250.14207911139789234No Hit
CTACTATTGCTATTCAAGACACAGCGATACAAGAGACATGGTTTTTGGCG16160.1412922117039963No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC16120.14094247850670918No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTG16100.14076761190806564No Hit
ACCCTCCGGCATCCCTGAGAGATTCTCTGGCTCCAGCTCGGGGACGATGGCCACCTTGACTATCGCTGGGGCCC15730.13753257983315978No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15720.13744514653383802No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15600.13639594694197663No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15460.1351718807514717No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG15380.1344724143568975No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15190.13281118166978367No Hit
GCTTGGTCCCTCCGCCAAAAACCATGTCTCTTGTATCGCTGTGTCTTGAATAGCAATAGTAGTCAGCTTCATCCT15020.1313248155813134No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14960.13080021578538273No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC14660.12817721680572933No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14540.12712801721386796No Hit
GACTACTATTGCTATTCAAGACACAGCGATACAAGAGACATGGTTTTTGG14500.12677828401658084No Hit
TCCTCTACCTGGGCCCCAGCGATAGTCAAGGTGGCCATCGTCCCCGAGCTGGAGCCAGAGAATCTCTCAGGGATG14400.12590395102336305No Hit
GTTTATTGGTATCAGCAGAAGTCAGGCCAGGCCCCTGTGTTGGTCATCAGTGAGAACACCAAGCGACCCTCCGGC14250.12459245153353636No Hit
AGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGG14220.12433015163557101No Hit
GCTTCATCCTCTACCTGGGCCCCAGCGATAGTCAAGGTGGCCATCGTCCCCGAGCTGGAGCCAGAGAATCTCTC13870.12126998615930873No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT13760.12030821986676914No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13700.11978362007083847No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13620.11908415367626421No Hit
GCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCAC13610.11899672037694244No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13600.11890928707762066No Hit
GGAGATGCATTGGCAAAGAGGTATGTTTATTGGTATCAGCAGAAGTCAGG13420.11733548768982861No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC13260.11593655490068015No Hit
GGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGG13220.11558682170339303No Hit
AGTCAGGCCAGGCCCCTGTGTTGGTCATCAGTGAGAACACCAAGCGACCC13120.11471248871017523No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA13090.11445018881220989No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGG12860.11243922292780895No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCAC12750.11147745663526937No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG12580.1099910905467991No Hit
GACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGGCAA12350.10798012466239817No Hit
GACTATCGCTGGGGCCCAGGTAGAGGATGAAGCTGACTACTATTGCTATT12170.10640632527460614No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC11970.10465765928817053No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAG11890.1039581928935963No Hit
ATAGTAGTCAGCTTCATCCTCTACCTGGGCCCCAGCGATAGTCAAGGTGGCCATCGTCCCCGAGCTGGAGCCAG11860.10369589299563096No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG11820.10334615979834384No Hit
GAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAG11740.10264669340376958No Hit
CAGGTAGAGGATGAAGCTGACTACTATTGCTATTCAAGACACAGCGATACAAGAGACATGGTTTTTGGCGGAGGG11550.10098546071665579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)