FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004718000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143729
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC80760.7061113253226945No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCAAAAACCAT69330.6061750641979No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC65520.5728629771563019No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40730.3561158281376095No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA39820.34815939789932754No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC35190.3076777803133435No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC34110.2982349839865912No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32990.2884424544625519No Hit
CCCCTGTGTTGGTCATCAGTGAGAACACCAAGCGACCCTCCGGCATCCCT32970.2882675878639083No Hit
GTGTTGGTCATCAGTGAGAACACCAAGCGACCCTCCGGCATCCCTGAGAGATTCTCTGGCTCCAGCTCGGGGACG32610.28511998908832425No Hit
ATTCAAGACACAGCGATACAAGAGACATGGTTTTTGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGG31340.2740159600744582No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC30410.2658846632375327No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT30380.26562236333956735No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG29990.2622124646660179No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG29580.2586276993938249No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG29020.25373143463180525No Hit
GTCTTGAATAGCAATAGTAGTCAGCTTCATCCTCTACCTGGGCCCCAGCGATAGTCAAGGTGGCCATCGTCCCCG28640.25040896925757766No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC28560.24970950286300342No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28390.24822313677453312No Hit
GTGAGAACACCAAGCGACCCTCCGGCATCCCTGAGAGATTCTCTGGCTCCAGCTCGGGGACGATGGCCACCTTG27710.2422776724206521No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC27100.2369442411620235No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG26990.23598247486948395No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCAAAAACCATGTCTCTTGTATCGCTGTGTCTTGAATAGCAATAGT26780.23414637558372658No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCAAAAACCATGTCTCTTGTATCGCTGTGTCTTGAATAGCAATAGT26730.23370920908711768No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25640.22417897946104365No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25600.22382924626375653No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC25190.22024448099156355No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25180.22015704769224176No Hit
ACACAGGGGCCTGGCCTGACTTCTGCTGATACCAATAAACATACCTCTTT24400.21333725034514295No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG24220.2117634509573509No Hit
CTCTTGTATCGCTGTGTCTTGAATAGCAATAGTAGTCAGCTTCATCCTCT23390.20450648711364317No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22810.19943535575297996No Hit
ATACAAGAGACATGGTTTTTGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTC22520.1968997900726483No Hit
GCTCTGGAGATGCATTGGCAAAGAGGTATGTTTATTGGTATCAGCAGAAG22410.19593802378010874No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC22190.1940144911950296No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG21880.1913040589160544No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGGCAAAGAGGTATGTTTATTGGTATCAGCAGA21710.18981769282758415No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC21430.18736956044657432No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC21410.18719469384793078No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA20560.17976286340557948No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20480.1790633970110052No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA20460.17888853041236166No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG20210.17670269792931717No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTC19890.17390483235102022No Hit
ACCTGGGCCCCAGCGATAGTCAAGGTGGCCATCGTCCCCGAGCTGGAGCC19590.17128183337136682No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19540.1708446668747579No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19390.16953316738493124No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC18770.1641123028269809No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18690.16341283643240662No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18340.16035267095614433No Hit
GGATGAAGCTGACTACTATTGCTATTCAAGACACAGCGATACAAGAGACATGGTTTTTGGCGGAGGGACCA18220.15930347136428297No Hit
GTGTTCTCACTGATGACCAACACAGGGGCCTGGCCTGACTTCTGCTGATA18070.15799197187445627No Hit
GGCCAGGCCCCTGTGTTGGTCATCAGTGAGAACACCAAGCGACCCTCCGGCATCCCTGAGAGATTCTCTGGCTCC17830.15589357269073356No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCAAAAACCATGT17350.15169677432328812No Hit
GGCCCAGGTAGAGGATGAAGCTGACTACTATTGCTATTCAAGACACAGCGATACAAGAGACATGGTTTTTGGCGG17350.15169677432328812No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC17150.14994810833685251No Hit
GGATCACCTGCTCTGGAGATGCATTGGCAAAGAGGTATGTTTATTGGTATCAGCAGAAGTCAGGCCAGGCCC16910.1478497091531298No Hit
GAGGTATGTTTATTGGTATCAGCAGAAGTCAGGCCAGGCCCCTGTGTTGGTCATCAGTGAGAACACCAAGCGACC16860.1474125426565209No Hit
GACCAACACAGGGGCCTGGCCTGACTTCTGCTGATACCAATAAACATACCTCTTTGCCAATGCATCTCCAGAGC16680.14583874326872887No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16600.14513927687415462No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTG16550.14470211037754574No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16450.14382777738432795No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16380.14321574428907546No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA16130.14102991180603097No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15960.13954354571756072No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTC15820.13831947952705578No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15760.13779487973112511No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15610.1364833802412984No Hit
GGTAGAGGATGAAGCTGACTACTATTGCTATTCAAGACACAGCGATACAAGAGACATGGTTTTTGGCGGAGGGAC15540.13587134714604596No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15440.13499701415282817No Hit
CTACTATTGCTATTCAAGACACAGCGATACAAGAGACATGGTTTTTGGCG14910.13036304928877382No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14640.12800235020708578No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC14620.1278274836084422No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG14590.12756518371047687No Hit
ACCCTCCGGCATCCCTGAGAGATTCTCTGGCTCCAGCTCGGGGACGATGGCCACCTTGACTATCGCTGGGGCCC14440.12625368422065017No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14440.12625368422065017No Hit
GCTTGGTCCCTCCGCCAAAAACCATGTCTCTTGTATCGCTGTGTCTTGAATAGCAATAGTAGTCAGCTTCATCCT14410.12599138432268484No Hit
GTTTATTGGTATCAGCAGAAGTCAGGCCAGGCCCCTGTGTTGGTCATCAGTGAGAACACCAAGCGACCCTCCGGC13700.11978362007083847No Hit
GACTACTATTGCTATTCAAGACACAGCGATACAAGAGACATGGTTTTTGG13570.11864698717965533No Hit
GCTTCATCCTCTACCTGGGCCCCAGCGATAGTCAAGGTGGCCATCGTCCCCGAGCTGGAGCCAGAGAATCTCTC13420.11733548768982861No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13410.11724805439050684No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCAAAAACC13370.11689832119321972No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13210.11549938840407124No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC13070.11427532221356632No Hit
GCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCAC13060.11418788891424454No Hit
AGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGG12800.11191462313187826No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC12730.1113025900366258No Hit
TCCTCTACCTGGGCCCCAGCGATAGTCAAGGTGGCCATCGTCCCCGAGCTGGAGCCAGAGAATCTCTCAGGGATG12690.11095285683933868No Hit
GGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGG12690.11095285683933868No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA12660.11069055694137335No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC12610.11025339044476445No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC12190.1065811918732497No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT12170.10640632527460614No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12110.10588172547867546No Hit
GGAGATGCATTGGCAAAGAGGTATGTTTATTGGTATCAGCAGAAGTCAGG12050.10535712568274476No Hit
GTATCAACGCAGAGTACGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTC11840.10352102639698739No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG11820.10334615979834384No Hit
GACTATCGCTGGGGCCCAGGTAGAGGATGAAGCTGACTACTATTGCTATT11790.1030838599003785No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCAAAAAC11620.10159749381190825No Hit
AGTCAGGCCAGGCCCCTGTGTTGGTCATCAGTGAGAACACCAAGCGACCC11580.10124776061462112No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGG11520.10072316081869044No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11440.10002369442411621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)