FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004718001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325979
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCTTCAGGGAGAACTGCTTTCTGGACGTGTCTACTG35320.2663692260586329No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGATCACCATCTCCTG33160.25007937531439034No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGAGTACTACTGTATGTATATGAGGTGCAGTAATAAT32710.24668565640933982No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32330.2438198493339638No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG32170.24261319372327916No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGAGTACTACTGTATGTATATGAGGTG31750.23944572274523201No Hit
CTCCTGCACTGGCACCAGCGGTGACATTGGTGCTTATAACTCTGTCTCCTGGTACCAACAGTACCCTGGCAAAGC29360.2214213045606303No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27360.20633810942707237No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG26980.20347230235169636No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA26270.1981177680792833No Hit
GTCTCTGGTGACTCCATCAGTGGTTTCTACTGGAACTGGATCCGGCAGCC25140.1895957628288231No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC24810.18710703563178602No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC24360.1837133167267355No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC23530.17745379074630896No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22910.172778000254906No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22580.17028927305786895No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22280.16802679378783525No Hit
CAGTAGTACTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTC22020.16606597842047272No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG21740.1639543311017746No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG21250.16025894829405293No Hit
TCTCTGGGCTCCGGGCTGAGGACGAGGCTGATTATTACTGCACCTCATAT20750.15648814951066345No Hit
GTACTACTGTATGTATATGAGGTGCAGTAATAATCAGCCTCGTCCTCAGC20550.15497982999730767No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT20170.15211402292193166No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGTCAGACTTGGAGGCAGAGAAGCGATCAGAAACCCCTGAGGGCCGAT19940.15037945548157247No Hit
CTCCTGGGCAGTCGATCACCATCTCCTGCACTGGCACCAGCGGTGACATTGGTGCTTATAACTCTGTCTCCTGGT19830.1495498797492268No Hit
GTCTATTACTGTGCGAGAGGGTCGTCTTGTAGTGGTGCCACTTGCTACAATTTAGCCTTTTTGAGCCCCTGGGGC19830.1495498797492268No Hit
ATGTATATGAGGTGCAGTAATAATCAGCCTCGTCCTCAGCCCGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGT19810.14939904779789123No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG19670.14834322413854217No Hit
GAGTACTACTGTATGTATATGAGGTGCAGTAATAATCAGCCTCGTCCTCAGCCCGGAGCCCAGAGATGGTCAGGG19660.14826780816287438No Hit
GTAATAATCAGCCTCGTCCTCAGCCCGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGTCAGACTTGGAGGCAG19630.148041560235871No Hit
GATTATTACTGCACCTCATATACATACAGTAGTACTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT19470.14683490462518636No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18930.14276244193912574No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC18640.14057537864475983No Hit
CTACAAGACGACCCTCTCGCACAGTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA18460.1392178910827396No Hit
CATATACATACAGTAGTACTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCC18400.13876539522873288No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC18150.13687999583703814No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17980.13559792425068573No Hit
GTAGTACTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT17890.1349191804696756No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGATCAC17730.13371252485899096No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGATCACCATCT17300.13046963790527602No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16910.12752841485423222No Hit
CACCTGCACTGTCTCTGGTGACTCCATCAGTGGTTTCTACTGGAACTGGATCCGGCAGCCCGCCGGGAAGGG16680.1257938474138731No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG16480.12428552790051729No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16020.12081639301979895No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGAGTACTACTGTATGTATATGAGGTGCAGTAATAATC15460.11659309838240274No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15430.11636685045539938No Hit
ACAGTACCCTGGCAAAGCCCCCAAACTCATGATTTATGATGTCACCTATCGGCCCTCAGGGGTTTCTGATCGCTT15410.1162160185040638No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC15320.11553727472305368No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA15240.11493394691771137No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA15020.11327479545302001No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14930.11259605167200988No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGATCAC14590.11003190849930504No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC14380.10844817301028145No Hit
GTCTTGTAGTGGTGCCACTTGCTACAATTTAGCCTTTTTGAGCCCCTGGGGCCCGGGAACCCTGGTCACCGTCTC14280.10769401325360357No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC14150.1067136055699223No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14050.1059594458132444No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATC14010.10565778191057323No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13920.10497903812956315No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCTTGTATTTCCAGGAGAAAGTGATGG13860.1045265422755564No Hit
GTATGTATATGAGGTGCAGTAATAATCAGCCTCGTCCTCAGCCCGGAGCC13720.10347071861620735No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT13630.10279197483519724No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13610.10264114288386166No Hit
GTCGATCACCATCTCCTGCACTGGCACCAGCGGTGACATTGGTGCTTATAACTCTGTCTCCTGGTACCAACAGT13560.10226406300552272No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT13550.10218864702985493No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGATCACCATCTCCTGCACTGGCACC13500.10181156715151597No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)