FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004718002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325979
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGATCACCATCTCCTG33060.24932521555771245No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCTTCAGGGAGAACTGCTTTCTGGACGTGTCTACTG32450.24472484104197728No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32030.24155737006393013No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC29310.22104422468229137No Hit
CTCCTGCACTGGCACCAGCGGTGACATTGGTGCTTATAACTCTGTCTCCTGGTACCAACAGTACCCTGGCAAAGC29300.22096880870662358No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGAGTACTACTGTATGTATATGAGGTG28990.2186309134609221No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28900.217952169679912No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGAGTACTACTGTATGTATATGAGGTGCAGTAATAAT28890.2178767537042442No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27850.2100334922347941No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA26920.20301980649768964No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25600.1930648977095414No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC23960.1806966777000239No Hit
GTCTCTGGTGACTCCATCAGTGGTTTCTACTGGAACTGGATCCGGCAGCC22960.17315508013324496No Hit
CAGTAGTACTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTC21220.16003270036704956No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20940.15792105304835144No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC20580.15520607792431101No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20350.15347151048395186No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20050.15120903121391818No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGATCAC20020.1509827832869148No Hit
GTACTACTGTATGTATATGAGGTGCAGTAATAATCAGCCTCGTCCTCAGC19930.15030403950590468No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT19830.1495498797492268No Hit
TCTCTGGGCTCCGGGCTGAGGACGAGGCTGATTATTACTGCACCTCATAT19770.14909738389522004No Hit
CTCCTGGGCAGTCGATCACCATCTCCTGCACTGGCACCAGCGGTGACATTGGTGCTTATAACTCTGTCTCCTGGT19650.1481923921872066No Hit
ATGTATATGAGGTGCAGTAATAATCAGCCTCGTCCTCAGCCCGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGT19540.1473628164548609No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19470.14683490462518636No Hit
GAGTACTACTGTATGTATATGAGGTGCAGTAATAATCAGCCTCGTCCTCAGCCCGGAGCCCAGAGATGGTCAGGG19100.14404451352547815No Hit
GATTATTACTGCACCTCATATACATACAGTAGTACTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT18980.14313952181746467No Hit
GTCTATTACTGTGCGAGAGGGTCGTCTTGTAGTGGTGCCACTTGCTACAATTTAGCCTTTTTGAGCCCCTGGGGC18900.14253619401212236No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG18660.1407262105960954No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGTCAGACTTGGAGGCAGAGAAGCGATCAGAAACCCCTGAGGGCCGAT18510.1395949709610786No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18160.13695541181270593No Hit
GTAGTACTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT17820.1343912686400011No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17820.1343912686400011No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC17580.13258128522397414No Hit
CATATACATACAGTAGTACTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCC17520.1321287893699674No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC17090.12888590241625245No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16910.12752841485423222No Hit
GTAATAATCAGCCTCGTCCTCAGCCCGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGTCAGACTTGGAGGCAG16900.12745299887856446No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG16760.1263971752192154No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC16620.12534135155986634No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGATCACCATCT16580.12503968765719517No Hit
CTACAAGACGACCCTCTCGCACAGTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA15950.12028848119012443No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC15600.1176489220417518No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15210.114707698990708No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATC15050.11350104338002337No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15010.11319937947735222No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG14910.11244521972067431No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGATCAC14830.111841891915332No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14670.11063523630464736No Hit
CACCTGCACTGTCTCTGGTGACTCCATCAGTGGTTTCTACTGGAACTGGATCCGGCAGCCCGCCGGGAAGGG14330.10807109313194252No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA14060.10603486178891218No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC13910.10490362215389536No Hit
ACAGTACCCTGGCAAAGCCCCCAAACTCATGATTTATGATGTCACCTATCGGCCCTCAGGGGTTTCTGATCGCTT13750.10369696654321071No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13730.10354613459187514No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA13570.1023394789811905No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGC13530.10203781507851935No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13460.10150990324884482No Hit
GTCGATCACCATCTCCTGCACTGGCACCAGCGGTGACATTGGTGCTTATAACTCTGTCTCCTGGTACCAACAGT13320.10045407958949576No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)