FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004718017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692600
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCGCGGCAGTCACAGACCTCAGACTCAGGGAGA30790.4445567427086341No Hit
GTCTCTGGTGTCTCCATCACCAGTGGTGACTACTGCTGGACCTGGATCCG21160.30551544903263067No Hit
GTCTATTACTGTGCGAGGCAGGTGACGTGGGTATTACTACGAGGCTTTGACTACTGGGGCCAGGGAAAGCTGGTC18720.2702858792954086No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18220.26306670516892866No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18100.2613341033785735No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT17160.2477620560207912No Hit
ATTACAACCCGTCCCTCAAGAGTCGCCTTTCCATATCACTTGACATGTCTCAGAATCAATTCTCCCTGAGTCTG14110.20372509384926366No Hit
CTCTTATGCTGTAGACAGTAATAAGTTGCAGAGTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAACTCTGTC13750.1985272884781981No Hit
GCATAAGAGTTACCCGCTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCAT13730.1982385215131389No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC13110.1892867455963038No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12940.1868322263933006No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12070.17427086341322554No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12030.17369332948310715No Hit
GTCCTGGACCTCCTGTGCAAGAGCATGAAACACCTGTGGCTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC11770.16993935893733755No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT11200.16170950043315044No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCC10810.1560785446144961No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10800.15593416113196648No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG10440.15073635576090097No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTAGCAGTGATTTAGGCTGGTATCAGCAGA10060.1452497834247762No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG10030.14481663297718742No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9980.1440947155645394No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9520.13745307536817788No Hit
GTGCAAGAGCATGAAACACCTGTGGCTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTCCTGTCCCAGGTGC9340.1348541726826451No Hit
CAGTAATAGACGGCCGTGTCCGCGGCAGTCACAGACCTCAGACTCAGGGA9140.13196650303205312No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG9120.13167773606699393No Hit
GACCTACACCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA8930.12893444989893155No Hit
TGTGTGAGTTGTGTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATT8840.12763499855616517No Hit
CCCTATCCCTCACGTGCACTGTCTCTGGTGTCTCCATCACCAGTGGTGAC8700.1256136298007508No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA8550.12344787756280681No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTT8370.12084897487727403No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG8340.12041582442968524No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT8210.11853883915680047No Hit
GATTTAGGCTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAACCGTCTGAT8150.11767253826162286No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGGGGAAGACCGATGGGCCCTTGGTGGA8060.11637308691885648No Hit
ACTTATTACTGTCTACAGCATAAGAGTTACCCGCTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGA8050.11622870343632688No Hit
CACGTGCACTGTCTCTGGTGTCTCCATCACCAGTGGTGACTACTGCTGGA8000.11550678602367889No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7980.1152180190586197No Hit
GTGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCTGAGGGAGTAGAGTCCTGAGGACTGT7910.1142073346809125No Hit
GTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAGCCTAAATCACTGCTAA7900.1140629511983829No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA7790.11247473289055733No Hit
CCGTGGGCATGTGTGAGTTGTGTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCACCTTGGTGTTGCTGGG7760.11204158244296851No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGTGGACGGCGTGGAGGTGCATAATGCC7760.11204158244296851No Hit
GGACTGGTGAGGCCTTCACAGACCCTATCCCTCACGTGCACTGTCTCTGGTGTCTCCATCACCAGTGGTGACT7570.10929829627490614No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTT7540.10886514582731735No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC7510.10843199537972856No Hit
ACCTATTACAACCCGTCCCTCAAGAGTCGCCTTTCCATATCACTTGACATGTCTCAGAATCAATTCTCCCTGAGT7500.10828761189719896No Hit
GTAATACCCACGTCACCTGCCTCGCACAGTAATAGACGGCCGTGTCCGCGGCAGTCACAGACCTCAGACTCAGGG7470.10785446144961017No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA7350.10612185965925497No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGAC7310.10554432572913658No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACATCC7270.10496679179901819No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG7260.10482240831648859No Hit
GGTTGTAATAGGTACCCCCACTGTGGAAAGAGATGTATCCAATCCACTCC7260.10482240831648859No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGG7190.10381172393878141No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC7180.10366734045625181No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7180.10366734045625181No Hit
CTATTACTGTGCGAGGCAGGTGACGTGGGTATTACTACGAGGCTTTGACTACTGGGGCCAGGGAAAGCTGGTC7100.10251227259601502No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT7080.10222350563095582No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7070.10207912214842622No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)