FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004718018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692600
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCGCGGCAGTCACAGACCTCAGACTCAGGGAGA28290.4084608720762345No Hit
GTCTCTGGTGTCTCCATCACCAGTGGTGACTACTGCTGGACCTGGATCCG20080.289922032919434No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17190.24819520646838003No Hit
GTCTATTACTGTGCGAGGCAGGTGACGTGGGTATTACTACGAGGCTTTGACTACTGGGGCCAGGGAAAGCTGGTC17150.2476176725382616No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15680.22639330060641064No Hit
CTCTTATGCTGTAGACAGTAATAAGTTGCAGAGTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAACTCTGTC14110.20372509384926366No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT13640.19693907017037252No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT13620.19665030320531332No Hit
ATTACAACCCGTCCCTCAAGAGTCGCCTTTCCATATCACTTGACATGTCTCAGAATCAATTCTCCCTGAGTCTG13570.19592838579266533No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13280.19174126479930698No Hit
GCATAAGAGTTACCCGCTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCAT12370.1786023678891135No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11460.16546347097892003No Hit
GTCCTGGACCTCCTGTGCAAGAGCATGAAACACCTGTGGCTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC11360.16401963615362403No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10960.15824429685244007No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10810.1560785446144961No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10690.1543459428241409No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA9970.1439503320820098No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGGGGAAGACCGATGGGCCCTTGGTGGA9920.14322841466936181No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCC9890.14279526422177302No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9100.13138896910193473No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8800.12705746462604678No Hit
GTGCAAGAGCATGAAACACCTGTGGCTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTCCTGTCCCAGGTGC8700.1256136298007508No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG8580.1238810280103956No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTAGCAGTGATTTAGGCTGGTATCAGCAGA8530.12315911059774762No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG8440.12185965925498124No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8370.12084897487727403No Hit
GTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAACTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC8250.11911637308691886No Hit
TGTGTGAGTTGTGTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATT8060.11637308691885648No Hit
CCCTATCCCTCACGTGCACTGTCTCTGGTGTCTCCATCACCAGTGGTGAC8040.11608431995379728No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT8030.11593993647126768No Hit
CAGTAATAGACGGCCGTGTCCGCGGCAGTCACAGACCTCAGACTCAGGGA7870.11362980075079411No Hit
ACTTATTACTGTCTACAGCATAAGAGTTACCCGCTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGA7840.11319665030320532No Hit
CACGTGCACTGTCTCTGGTGTCTCCATCACCAGTGGTGACTACTGCTGGA7770.11218596592549811No Hit
ACCTATTACAACCCGTCCCTCAAGAGTCGCCTTTCCATATCACTTGACATGTCTCAGAATCAATTCTCCCTGAGT7700.11117528154779092No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA7700.11117528154779092No Hit
GATTTAGGCTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAACCGTCTGAT7350.10612185965925497No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC7340.10597747617672537No Hit
GTGTTGTAGTTGTTCTCCGGCTGCCCGCTGCTCTCCCACTCCACGGCGATGTCGCTGGGGTAGAAGCCTTTGACC7340.10597747617672537No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7320.10568870921166618No Hit
GGTTGTAATAGGTACCCCCACTGTGGAAAGAGATGTATCCAATCCACTCC7290.10525555876407738No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTT7190.10381172393878141No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG7180.10366734045625181No Hit
GTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAGCCTAAATCACTGCTAA7150.103234190008663No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTT7140.1030898065261334No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG7120.10280103956107421No Hit
GCCCCTAACCGTCTGATCTACGCTGCTTCCAGTCTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT6980.10077967080565985No Hit
CTATTACTGTGCGAGGCAGGTGACGTGGGTATTACTACGAGGCTTTGACTACTGGGGCCAGGGAAAGCTGGTC6940.10020213687554143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)