FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004718021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952321
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTTACACAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC49510.5198877269324104No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40160.4217065464270976No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT35750.3753986313438431No Hit
GTGTAAAGTAGACGCCTTGTAGATCAGGAGCTGAGGGGCTTTCCCTGGTTTGTGCTGATACCAGGCCAACCAGGT35460.3723534396490259No Hit
CCTTCCACACTGTCTGCATCTGTAGGAGACAGAGTCACCATCTCTTGCCG26660.2799476227028491No Hit
GTATTAGTACCTGGTTGGCCTGGTATCAGCACAAACCAGGGAAAGCCCCTCAGCTCCTGATCTACAAGGCGTCT25340.26608675016092265No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCC25250.2651416906694276No Hit
GAGTATTAGTACCTGGTTGGCCTGGTATCAGCACAAACCAGGGAAAGCCCCTCAGCTCCTGATCTACAAGGCGT24520.2574762081273016No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24430.2565311486358066No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCTCTTGCCGGGCCAGTCAGA24350.25569109575447774No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23860.25054577185633836No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG23070.24225024965321565No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG22670.23804998524657128No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21020.2207238945691631No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19900.20896315423055883No Hit
ACCCTACAGTGACTACTGATCATGATCCTTTTGATATGTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGCAT18350.19268712965481177No Hit
GTGCAGCCACAGTTCGTTTCATCCCCACCTTGGTCCCTCCGCCGAAAGTGGAATAACTATGATACTCTTGGCAGA18020.18922191151933013No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA17640.18523166033301797No Hit
CTATGATACTCTTGGCAGAAGTAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC17490.1836565611805263No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG16680.1751510257570714No Hit
CTGTAGGGTAGTCTCTCACACAATAATAAACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA16580.1741009596554103No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA16570.1739959530452442No Hit
GAGTATCATAGTTATTCCACTTTCGGCGGAGGGACCAAGGTGGGGATGAAACGAACTGTGGCTGCACCAT16310.17126578118092534No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC16110.16916564897760314No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCACATGTGACATCC15810.16601545067261983No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT15690.16475537135062654No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCACATGTGACAT15650.16433534490996207No Hit
ATGATACTCTTGGCAGAAGTAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCC15420.16192019287614154No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA15250.1601350805033177No Hit
GTAGGAGACAGAGTCACCATCTCTTGCCGGGCCAGTCAGAGTATTAGTACCTGGTTGGCCTGGTATCAGCACAA14860.1560398227068394No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14660.1539396905035172No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCCCCACCTTGGTCCCTCCGCCGAAAGTGGAATAACTATG14010.14711426084272006No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13890.1458541815207267No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTG13330.13997381135142456No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13200.13860872541926514No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13070.13724363948710572No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT12830.13472348084311908No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12680.13314838169062743No Hit
AACCAGGGAAAGCCCCTCAGCTCCTGATCTACAAGGCGTCTACTTTACAC12370.12989317677547801No Hit
CTTCCACACTGTCTGCATCTGTAGGAGACAGAGTCACCATCTCTTGCCGG12360.1297881701653119No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12340.1295781569449797No Hit
GTACTAATACTCTGACTGGCCCGGCAAGAGATGGTGACTCTGTCTCCTACAGATGCAGACAGTGTGGAAGG12300.12915813050431524No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12260.1287381040636508No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12110.12716300491115914No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC12060.1266379718603286No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC12050.1265329652501625No Hit
GTATCAGCACAAACCAGGGAAAGCCCCTCAGCTCCTGATCTACAAGGCGTCTACTTTACACAGTGGGGTCCCATC12050.1265329652501625No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11990.12590292558916583No Hit
GTATCATAGTTATTCCACTTTCGGCGGAGGGACCAAGGTGGGGATGAAACGAACTGTGGCTGCACCATCTGTCTT11900.12495786609767086No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT11790.12380279338584364No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC11770.12359278016551142No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCACATGTGACATCCAGATGACCCAGTCTCCTTCCAC11730.12317275372484698No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11700.12285773389434866No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAC11320.11886748270803647No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10860.11403717864039542No Hit
GATCTACAAGGCGTCTACTTTACACAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTC10780.11319712575906654No Hit
ATCTATAGCAGTGGGGCCACCAAGTACAACCCCTCCCTACAGAGTCGAGTCACCATGTCAGTAGACATCTCTAAA10770.11309211914890041No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10690.11225206626757155No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10650.1118320398269071No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTACTTCTGCCAAGAGTATCATAGTTATTCCACTTTCGG10590.11120200016591045No Hit
CTAATACTCTGACTGGCCCGGCAAGAGATGGTGACTCTGTCTCCTACAGA10380.10899686135242215No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10310.10826181508125936No Hit
CCTTGGTCCCTCCGCCGAAAGTGGAATAACTATGATACTCTTGGCAGAAGTAAGTTGCAAAATCATCAGGCTGC10220.10731675558976438No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10140.10647670270843551No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10030.10532162999660828No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC9930.1042715638949472No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9920.10416655728478108No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9890.10385153745428273No Hit
GTCTCTGGTGGCTCCATTAACTATTACTACTGGAGTTGGATTCGGCAGCC9740.1022764383017911No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9630.10112136558996387No Hit
ACCTTGGTCCCTCCGCCGAAAGTGGAATAACTATGATACTCTTGGCAGAAGTAAGTTGCAAAATCATCAGGCTGC9570.10049132592896723No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)