FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004718022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952321
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTTACACAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC46330.4864956248995874No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40630.42664185710490476No Hit
GTGTAAAGTAGACGCCTTGTAGATCAGGAGCTGAGGGGCTTTCCCTGGTTTGTGCTGATACCAGGCCAACCAGGT36400.38222406100464024No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT29620.3110295793120177No Hit
GTATTAGTACCTGGTTGGCCTGGTATCAGCACAAACCAGGGAAAGCCCCTCAGCTCCTGATCTACAAGGCGTCT24730.2596813469407899No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCC24100.253065930500325No Hit
CCTTCCACACTGTCTGCATCTGTAGGAGACAGAGTCACCATCTCTTGCCG23050.24204023643288344No Hit
GAGTATTAGTACCTGGTTGGCCTGGTATCAGCACAAACCAGGGAAAGCCCCTCAGCTCCTGATCTACAAGGCGT22970.24120018355155456No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22550.23678990592457794No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCTCTTGCCGGGCCAGTCAGA22400.23521480677208628No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22120.23227462168743523No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22090.2319596018569369No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA21630.22712929778929583No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG18550.19478726185813397No Hit
ACCCTACAGTGACTACTGATCATGATCCTTTTGATATGTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGCAT18060.18964193795999457No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17620.18502164711268573No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA15890.1668555035539487No Hit
CTATGATACTCTTGGCAGAAGTAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC15750.16538541101162318No Hit
GTGCAGCCACAGTTCGTTTCATCCCCACCTTGGTCCCTCCGCCGAAAGTGGAATAACTATGATACTCTTGGCAGA15690.16475537135062654No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC15630.16412533168962987No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT15600.16381031185913153No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG15600.16381031185913153No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG15270.1603450937236499No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTG15080.15834996813049382No Hit
CTGTAGGGTAGTCTCTCACACAATAATAAACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA14890.15635484253733772No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14870.15614482931700552No Hit
GAGTATCATAGTTATTCCACTTTCGGCGGAGGGACCAAGGTGGGGATGAAACGAACTGTGGCTGCACCAT14670.1540446971136833No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCACATGTGACATCC14310.15026445914770334No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCACATGTGACAT14220.14931939965620836No Hit
ATGATACTCTTGGCAGAAGTAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCC14140.14847934677487948No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCCCCACCTTGGTCCCTCCGCCGAAAGTGGAATAACTATG14100.14805932033421504No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC13210.13871373202943124No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT13070.13724363948710572No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13050.1370336262667735No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12880.13524851389394962No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12850.1349334940634513No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12770.1340934411821224No Hit
GTAGGAGACAGAGTCACCATCTCTTGCCGGGCCAGTCAGAGTATTAGTACCTGGTTGGCCTGGTATCAGCACAA12760.1339884345719563No Hit
GTATCATAGTTATTCCACTTTCGGCGGAGGGACCAAGGTGGGGATGAAACGAACTGTGGCTGCACCATCTGTCTT12440.1306282230466408No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC12420.13041820982630856No Hit
AACCAGGGAAAGCCCCTCAGCTCCTGATCTACAAGGCGTCTACTTTACAC12130.12737301813149138No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA11990.12590292558916583No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11910.12506287270783698No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT11740.12327776033501309No Hit
GTACTAATACTCTGACTGGCCCGGCAAGAGATGGTGACTCTGTCTCCTACAGATGCAGACAGTGTGGAAGG11630.12212268762318587No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11540.12117762813169089No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCACATGTGACATCCAGATGACCCAGTCTCCTTCCAC11320.11886748270803647No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC11030.1158222910132193No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC11010.11561227779288707No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAC10980.11529725796238872No Hit
ATCTATAGCAGTGGGGCCACCAAGTACAACCCCTCCCTACAGAGTCGAGTCACCATGTCAGTAGACATCTCTAAA10740.11277709931840209No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10630.11162202660657489No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC10480.11004692745408323No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10370.10889185474225603No Hit
CTAATACTCTGACTGGCCCGGCAAGAGATGGTGACTCTGTCTCCTACAGA10310.10826181508125936No Hit
CTTCCACACTGTCTGCATCTGTAGGAGACAGAGTCACCATCTCTTGCCGG10140.10647670270843551No Hit
CCTTGGTCCCTCCGCCGAAAGTGGAATAACTATGATACTCTTGGCAGAAGTAAGTTGCAAAATCATCAGGCTGC10090.10595166965760494No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10060.1056366498271066No Hit
GTATCAGCACAAACCAGGGAAAGCCCCTCAGCTCCTGATCTACAAGGCGTCTACTTTACACAGTGGGGTCCCATC10040.1054266366067744No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9920.10416655728478108No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9910.10406155067461498No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9910.10406155067461498No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9910.10406155067461498No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC9710.10196141847129278No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTGTGTAAAGTAGACGCCTTGT9590.10070133914929945No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9570.10049132592896723No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)