FastQCFastQC Report
Wed 8 May 2024
EGAF00004718024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences897364
Total Bases66.2 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT42190.47015480897383893No Hit
GAGTTACACTATCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA42020.4682603714880472No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT37200.4145474968908938No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31010.34556768490824236No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28090.3130279351522905No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG25700.2863943728520422No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25400.2830512478771156No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT25280.281713997887145No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGCCTGCTCAGCTCCATGTAGG24540.27346762294899285No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24170.2693444354799167No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23630.2633268105250489No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22750.2535203105985977No Hit
GTAGGAGACAGAGTCACCATCTCTTGCCGGGCAAGTCAGAGCATTAGCAG22490.250622935620328No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21700.2418193731863547No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCTCTT20810.23190143576073924No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20490.22833543578748422No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG19910.22187206083595953No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA18700.2083881234370891No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC18270.20359631097302766No Hit
GTGTATTACTGTGCGAGGAGGTATCAACTACAGCACTACCAATACGGAAT17660.1967986235240103No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17660.1967986235240103No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17590.1960185610298608No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA16960.188997998582515No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16850.1877721860917086No Hit
GCATAGATCAGGAGCCTAGGGGCTTTCCCTGATTTCTGCTGATACCAATT16680.18587774860591688No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16440.18320324862597565No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16320.181865998636005No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA16310.1817545611368408No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG15980.17807712366442158No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15840.1765169986761225No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCT15770.17573693618197297No Hit
ATCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGT15720.17517974868615188No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC15370.17127943621540423No Hit
GCCTACATGGAGCTGAGCAGGCTGAGATCTGACGACACGGCCGTGTATTA15360.17116799871624No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAAC15310.1706108112204189No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC15110.16838206123713453No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14940.16648762375134282No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14770.1645931862655511No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA14610.1628101862789236No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14530.16191868628560985No Hit
ACACTATCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA14490.16147293628895298No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14420.16069287379480343No Hit
GGATAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGC14310.15946706130399704No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC14090.15701543632238424No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT14080.15690399882322No Hit
ACTTACTACTGTCAACAGAGTTACACTATCCCTCGGACGTTCGGCCAAGG13940.15534387383492096No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGA13820.15400662384495034No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG13680.15244649885665126No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCTCTTGCCGGGCAAGTCAGA13620.15177787386166594No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA13460.14999487387503846No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGT13440.14977199887671No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13290.14810043638924672No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC12840.14308574892685688No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12770.14230568643270736No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12660.14107987394190094No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12640.1408569989435725No Hit
GTGCATATAGTTGCCGGTGAAGGTGTATCCAGAAGCCTTGCAGGAGACCT12380.1379596239653028No Hit
TCCGAGGGATAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCA12260.1366223739753322No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12180.13573087398201844No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA12080.13461649899037625No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12040.1341707489937194No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG12030.13405931149455516No Hit
CTGTTAGGGTTGATCCATCCCATCCACTCAAGCCCTTGTCCAGGGGCCTG12020.13394787399539093No Hit
GATCAGGAGCCTAGGGGCTTTCCCTGATTTCTGCTGATACCAATTTAAAT12000.1337249989970625No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA11930.13294493650291297No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT11810.13160768651294236No Hit
CCCCTAGGCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCA11420.1272616240455378No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11420.1272616240455378No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT11410.1271501865463736No Hit
CCCTAACAGTGGTGGCACAAACTATGCACAGAACTTTCAGGGCAGGGTCA11390.12692731154804515No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC11200.124809999063925No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC11100.12369562407228282No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG11100.12369562407228282No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10940.12191262408565533No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10820.12057537409568471No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT10780.12012962409902782No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10640.11856949911072875No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC10570.11778943661657922No Hit
CAGTAATACACGGCCGTGTCGTCAGATCTCAGCCTGCTCAGCTCCATGTA10530.11734368661992237No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA10530.11734368661992237No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10160.11322049915084625No Hit
GTATTACTGTGCGAGGAGGTATCAACTACAGCACTACCAATACGGAATGG10140.11299762415251781No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10120.11277474915418939No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10060.11210612415920407No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACT10030.11177181166171142No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC9980.11121462416589033No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG9960.11099174916756188No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCA9930.11065743667006923No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA9850.10976593667675547No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG9800.10920874918093437No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC9700.10809437418929219No Hit
AGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAAATCAGGGAA9660.10764862419263532No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9590.1068685616984858No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGG9550.1064228117018289No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9440.10519699921102249No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACCCTAACAGTGGT9420.10497412421269407No Hit
GCCTAGGGGCTTTCCCTGATTTCTGCTGATACCAATTTAAATAGCTGCTA9420.10497412421269407No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC9370.10441693671687298No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTC9370.10441693671687298No Hit
CCATGTAGGCTGTGCTGATGGCCGTGTCCCTGGTCATGGTGACCCTGCCC9360.10430549921770875No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTGA9360.10430549921770875No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT9360.10430549921770875No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA9340.1040826242193803No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCATAGATCAGGAGC9290.10352543672355922No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9240.10296824922773813No Hit
ACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT9200.10252249923108127No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9120.1016309992377675No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9050.10085093674361796No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9040.10073949924445376No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCG9030.10062806174528954No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGA8990.10018231174863267No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)