FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004718031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1353337
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATAGTACTCCTCCACTTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT69560.5139887552028799No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62470.4615997345819999No Hit
ATTATAGTACTCCTCCACTTTTTGGCCAGGGGACCAAGCTGGAGATCAAA61990.4580529461619685No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGAGAAACCGGGGTCCCT57970.4283485931442058No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT55640.4111318910219702No Hit
GCTTAGGAGGCTGTCCTGGTCTCTGCTGATACCAAGCTAAGTAGTTCTTATTGTTGGAAATGTGTAAAACACT48850.36095961316360964No Hit
GACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGAGAA46220.3415261682788544No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40030.29578737594553317No Hit
GTACTATAATAGTGCTGACAGTAATAGACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT39350.29076275901715537No Hit
CTATAATAGTGCTGACAGTAATAGACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC39210.2897282790613129No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG37820.27945737092830536No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36150.26711750288361286No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35850.26490076012109326No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG34390.2541126120101645No Hit
GTCTATTACTGTCAGCACTATTATAGTACTCCTCCACTTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA33640.2485707551038655No Hit
GTCCTGGTCTCTGCTGATACCAAGCTAAGTAGTTCTTATTGTTGGAAATGTGTAAAACACTCTGGCTGGACTTGC33570.24805351512594423No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA32240.23822595554544063No Hit
GTAATAGACGGCCGTGTCTGCGGCAGTCACAGAGTTCACCTCCAGGGAGA32140.23748704129126744No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTAC31340.23157572725788184No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA30310.22396491043989783No Hit
CTATTACTGTCAGCACTATTATAGTACTCCTCCACTTTTTGGCCAGGGGA30220.22329988761114195No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG29870.22071368772153574No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC28950.21391567658314228No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC27460.20290585419596155No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT27180.20083689428427656No Hit
ATTCAGTGGCAGCGGGTTTGGGACAGATTTCACTCTCACCATCAGCAGCC25920.1915265746816942No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG25710.1899748547479305No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24510.18110788369785205No Hit
GTCTCTGGTGGCTCCATCAGTAATTACTACTGGGGCTGGATCCGGCAGCC24430.18051675229451347No Hit
GTTTTACACATTTCCAACAATAAGAACTACTTAGCTTGGTATCAGCAGAGACCAGGACAGCCTCCTAAGCTGCTC23830.17608326676947428No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAAGTGGAGGAGTACTATAATAGTGCTGAC23770.17563991821697034No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTACACATTTCCAACAATAAGAACT23760.17556602679155303No Hit
GTAATAGACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAAACCCGCTGCC23280.17201923837152164No Hit
TTATAGTACTCCTCCACTTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT23150.17105864984109648No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA22990.16987638703441937No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT22200.16403896442645105No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21500.15886656464723864No Hit
GTATCAGCAGAGACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGAGAAACCGGGGTCCCTG21450.15849710752015206No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTTTGGGACAGATTTCACTCTCAC21420.1582754332439001No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTACACATTTCCA21380.1579798675422308No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21300.15738873613889223No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21120.15605869048138046No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21110.15598479905596316No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC20680.15280746776301837No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20610.15229022778509715No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG20600.1522163363596798No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC19540.14438384526544387No Hit
GTCTATTACTGTGCGAGAAAGGCATATTGTGGTAGTGGCTGCTATTACTACTTTGACTCCTGGGGCCAGGGAACC19200.14187153680125497No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC19160.1415759710995857No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC19110.1412065139724991No Hit
CAGTAATAGACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAAACCCGCTGCC18810.1389897712099795No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTACACATTTCCAACA18680.1380291826795543No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC18670.137955291254137No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTCTATTACTGTCAGC18560.13714248557454647No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18210.13455628568494027No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18150.13411293713243633No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT18020.13315234860201117No Hit
GTCAGGGACCCCGGTTTCTCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTCTCTGCTGAT17940.13256121719867262No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC17830.1317484115190821No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17760.13123117154116085No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG17620.13019669158531835No Hit
GCTCATTTACTGGGCATCTACCCGAGAAACCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTTTGGGACAGATTT17590.1299750173090664No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTAATTACTACTGGGGCTGGATCCGGCAGCCCCCAGGGAAGGG16820.12428537755193275No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16800.1241375947010981No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTCTATTACTGTCAGCACTATTATAGTACT16740.12369424614859417No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTCTCTGCTGATACCAAGCTA16580.12251198334191704No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15770.11652677788311411No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC15740.11630510360686214No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT15600.11527062365101967No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15380.11364501229183863No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15340.11334944659016934No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15190.11224107520890954No Hit
CTGCTGATACCAAGCTAAGTAGTTCTTATTGTTGGAAATGTGTAAAACAC15030.1110588124022324No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTGTTTTACACATTTCCAACAATAAGAACTACTTAGCTTGGTATCAGC14670.10839872108720887No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCTTGTATTTCCAGGAGAAAGTGATGG14560.10758591540761836No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA14040.1037435612859177No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT13790.10189627565048469No Hit
GTGTAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGG13590.10041844714213828No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG13590.10041844714213828No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13580.10034455571672096No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)