FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004718033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702729
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23060.3281492581066101No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT20770.2955620160830135No Hit
GAGTAGTACTTTACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT20570.2927159687447081No Hit
GTAATACACGGCCGTGTCCTCGTCTCTCAGGCTGTTCATTAGAAGATATACTGTGTTCTTGGAAATGTCTCTGG20410.2904391308740638No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19950.28389322199596145No Hit
AGCTTACACTGGTACCAGCAGAAACCAGATCTGTCTCCAAGGCTCCTCATCAAGTCTGCTTCTCAGTCCTTGTC19950.28389322199596145No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG17330.24661000186416102No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC16280.23166825333805777No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16230.23095674150348144No Hit
GTATTACTGTCATCAGAGTAGTACTTTACCGTGGACGTTCGGCCAAGGGA15580.22170708765398894No Hit
GTACTACTCTGATGACAGTAATACGTTGCAGCATCTTCAGCTTCCAGGCCATTGATGGTGAGACTGAAATCTGTC15540.2211378781863279No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14330.20391929178958032No Hit
GTGTATTACTGTGCAAAGAGTGGCTACGACCGTAATTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT14230.20249626812042765No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG13850.19708877817764744No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13170.18741221722740914No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13010.18513537935676486No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCTTGTATTTCCAGGAGAAAGTGATGG12680.18043940124856098No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG12370.17602802787418764No Hit
CAGTAATACGTTGCAGCATCTTCAGCTTCCAGGCCATTGATGGTGAGACTGAAATCTGTCCCAGATCCACTGCC11860.16877060716150893No Hit
GTAAAGTACTACTCTGATGACAGTAATACGTTGCAGCATCTTCAGCTTCCAGGCCATTGATGGTGAGACTGAAAT11230.15980555804584698No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA11120.15824023200977902No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11020.15681720834062632No Hit
GTGTAAGCTACGACCAATGCTCTGACTGGCCCGGCAGGTGATGGTGACTTTCTCCTTTGGAGTCACAGACTGAA10940.15567878940530416No Hit
GTACTTTACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA10890.15496727757072784No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATC10650.15155202076476137No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTCCTTGTTCCTATTTTAAAAGGTGTCC10270.14614453082198117No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC10250.14585992608815063No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG10220.14543301898740482No Hit
TCTCAGTCCTTGTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGG10090.14358308821750632No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC10060.1431561811167605No Hit
ACCACATACTACGCAGACTCCGTGAGGGGCCGATTCACCATCTCCAGAGACATTTCCAAGAACACAGTATATCTT9990.14216006454835362No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT9830.13988322667770933No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGTAAAGTACTACTCTGATGAC9620.13689487697248867No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9260.131771991763539No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT9130.1299220609936405No Hit
CTCCTGTGCAGCCACTGGCTTCACCGTCAGTAGCAACTACATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG8910.1267914089215046No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC8910.1267914089215046No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGG8810.1253683852523519No Hit
GCCCAGCACTGGGATTCCGAGGTGTTTCCATTCGGTGATCAGCACTGAAC8690.12366075684936868No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC8490.12081470951106331No Hit
GTCCTTGTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCAGTCTCACCATCAATGGC8420.11981859294265641No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA8260.11754175507201212No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC8240.11725715033818157No Hit
GTATGTGGTATCACCGGTATAAATAACTGAGACCCACTCCAGCCCCTTCC8120.11554952193519835No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAG7980.11355728879838459No Hit
GTCTCCAGACTTTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACCT7910.11256117222997769No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGTAATTACGGTCGTAGCCACTCTTTGCAC7910.11256117222997769No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT7900.11241886986306245No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT7890.11227656749614717No Hit
CTCCAAGGCTCCTCATCAAGTCTGCTTCTCAGTCCTTGTCAGGGGTCCCC7600.10814979885560437No Hit
GTCTCCAAGGCTCCTCATCAAGTCTGCTTCTCAGTCCTTGTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGAT7580.10786519412177382No Hit
GCTTGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG7550.10743828702102802No Hit
GAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTCCTTGT7530.10715368228719749No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGTAAAGT7500.10672677518645168No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7500.10672677518645168No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7490.10658447281953641No Hit
GTATATCTTCTAATGAACAGCCTGAGAGACGAGGACACGGCCGTGTATTACTGTGCAAAGAGTGGCTACGACCGT7400.105303751517299No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT7400.105303751517299No Hit
CCCTGAGACTCTCCTGTGCAGCCACTGGCTTCACCGTCAGTAGCAACTAC7390.10516144915038372No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7340.10444993731580739No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7330.10430763494889211No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGCCAGAGCATGTTCACCTGCCGCGTGG7260.10331151838048522No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCAGTCTCACCATCAATGGCC7240.1030269136466547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)