FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004718034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702729
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22990.3271531415382032No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG22960.3267262344374574No Hit
GAGTAGTACTTTACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT19780.2814740817584019No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC19510.2776319178516896No Hit
GTAATACACGGCCGTGTCCTCGTCTCTCAGGCTGTTCATTAGAAGATATACTGTGTTCTTGGAAATGTCTCTGG19060.2712283113405025No Hit
AGCTTACACTGGTACCAGCAGAAACCAGATCTGTCTCCAAGGCTCCTCATCAAGTCTGCTTCTCAGTCCTTGTC18820.2678130545345361No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT18760.26695924033304447No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14580.20747685096246204No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14570.2073345485955468No Hit
GTACTACTCTGATGACAGTAATACGTTGCAGCATCTTCAGCTTCCAGGCCATTGATGGTGAGACTGAAATCTGTC14320.20377698942266506No Hit
GTATTACTGTCATCAGAGTAGTACTTTACCGTGGACGTTCGGCCAAGGGA13990.1990810113144612No Hit
GTGTATTACTGTGCAAAGAGTGGCTACGACCGTAATTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT13240.18840833379581604No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12490.17773565627717086No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12320.1753165160396113No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA11870.1689129095284242No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG11730.16692067639161043No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATC11520.16393232668638977No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCTTGTATTTCCAGGAGAAAGTGATGG11400.16222469828340655No Hit
CAGTAATACGTTGCAGCATCTTCAGCTTCCAGGCCATTGATGGTGAGACTGAAATCTGTCCCAGATCCACTGCC11230.15980555804584698No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC10660.15169432313167666No Hit
GTAAAGTACTACTCTGATGACAGTAATACGTTGCAGCATCTTCAGCTTCCAGGCCATTGATGGTGAGACTGAAAT10530.14984439236177816No Hit
GTACTTTACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA10520.14970208999486287No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10350.1472829497573033No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG10250.14585992608815063No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC10040.14287157638293No Hit
GTGTAAGCTACGACCAATGCTCTGACTGGCCCGGCAGGTGATGGTGACTTTCTCCTTTGGAGTCACAGACTGAA9910.1410216456130315No Hit
TCTCAGTCCTTGTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGG9780.13917171484313298No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC9740.13860250537547192No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTCCTTGTTCCTATTTTAAAAGGTGTCC9630.13703717933940396No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9510.13532955093642074No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT9380.13347962016652223No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG9340.13291041069886114No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG9310.13248350359811534No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGTAAAGTACTACTCTGATGAC9270.13191429413045427No Hit
ACCACATACTACGCAGACTCCGTGAGGGGCCGATTCACCATCTCCAGAGACATTTCCAAGAACACAGTATATCTT8960.12750292075608094No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT8480.12067240714414802No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGG8330.118537871640419No Hit
GTCCTTGTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCAGTCTCACCATCAATGGC7970.1134149864314693No Hit
CTCCTGTGCAGCCACTGGCTTCACCGTCAGTAGCAACTACATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG7930.11284577696380825No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT7910.11256117222997769No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7870.11199196276231661No Hit
GCCCAGCACTGGGATTCCGAGGTGTTTCCATTCGGTGATCAGCACTGAAC7740.11014203199241812No Hit
CTCCAAGGCTCCTCATCAAGTCTGCTTCTCAGTCCTTGTCAGGGGTCCCC7700.10957282252475706No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC7640.10871900832326543No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC7520.10701137992028222No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGCCAGAGCATGTTCACCTGCCGCGTGG7500.10672677518645168No Hit
GCTTGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG7490.10658447281953641No Hit
GTCTCCAGACTTTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACCT7420.10558835625112953No Hit
GTATGTGGTATCACCGGTATAAATAACTGAGACCCACTCCAGCCCCTTCC7360.10473454204963792No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC7270.1034538207474005No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC7260.10331151838048522No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7080.10075007577601038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)