FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004718039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376017
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATAACTAGGTGGTTAGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTGAGTTACTGATCTATGGTGCATC14320.38083384527827197No Hit
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACAGTGTGTTCTTGGAATTGTCTCTGG14270.3795041181648702No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12970.3449312132164237No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT11620.30902858115457543No Hit
GTGTATTACTGTGCGAGAGACCGAGTGGTGATAACTTCCAACTGGATCGACCCCTGGGGCCAGGGAACCCTGGTC8050.2140860652576878No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8040.21382011983500748No Hit
GATCTATGGTGCATCCAGTTTGCAAAGCGGGGTCCCATCAAGGTTCAGCGGCAGTGGGTCTGGGACAGATTTC7890.2098309384948021No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7630.20291635750511278No Hit
CTCTAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTACATAGCATTGCTTTTGAAGGTGAATCCAGAGGCTGCAC7560.20105473954635028No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC7340.1952039402473824No Hit
GGCTAACAGTTTCCCTATCACTTTCGGCCCTGGGACCAAAGTGGATGTCAAACGAACTGTGGCTGCACCAT7130.18961908637109492No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG6980.18562990503088955No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6940.18456612334016814No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG6570.1747261427009949No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG6560.17446019727831455No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6350.16887534340202703No Hit
GCTATGTACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTAGAGTGGGTGGCATTTATATCATATGATGGAAGGAAT6350.16887534340202703No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6300.16754561628862524No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT6030.1603650898762556No Hit
GGGCTAGAGTGGGTGGCATTTATATCATATGATGGAAGGAATAAAAACTACGCAGACTCCGTGAAGGGCCGATTC5900.15690779938141095No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5790.153982399731927No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5630.1497272729690413No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC5620.14946132754636093No Hit
CACTATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTTCCCTATCACTTT5600.14892943670100023No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATA5370.142812691979352No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATAGTGAGAGTGAAATCTGTC5300.14095107402058948No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA5210.13855756521646628No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC4960.13190892964945733No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA4960.13190892964945733No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG4880.12978136626801448No Hit
GTCTGGGACAGATTTCACTCTCACTATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGG4810.12791974830925196No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCC4780.1271219120412109No Hit
GTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCCCAGGGCCGAAAGTGATAGGGAAACTGTTAGCCTGTTGAC4770.12685596661853055No Hit
GTTATACCCTGACTCGCCCGACAAGTGATGGTGACTGTGTCTCCTACAGA4740.1260581303504895No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG4690.12472840323708768No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC4630.12313273070100553No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTG4630.12313273070100553No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC4620.12286678527832519No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG4610.12260083985564482No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG4600.12233489443296446No Hit
CCATAGATCAGTAACTCAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGC4580.12180300358760375No Hit
GTACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTAGAGTGGGTGGCATTTATATCATATGATGGAAGGAATAAAA4540.12073922189688231No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT4540.12073922189688231No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC4520.12020733105152161No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4500.1196754402061609No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG4480.11914354936080017No Hit
GTAGGAGACACAGTCACCATCACTTGTCGGGCGAGTCAGGGTATAACTAGGTGGTTAGCCTGGTATCAGCAG4420.11754787682471803No Hit
GACCAGGGAAAGCCCCTGAGTTACTGATCTATGGTGCATCCAGTTTGCAA4400.11701598597935732No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC4340.11542031344327518No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTG4320.11488842259791447No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG4320.11488842259791447No Hit
GTATTACTGTGCGAGAGACCGAGTGGTGATAACTTCCAACTGGATCGACCCCTGGGGCCAGGGAACCCTGGTCAC4300.11435653175255374No Hit
GAATAAAAACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACACTGTAT4280.11382464090719303No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC4210.11196302294843052No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCCCAGGGCCGAAAGTGATAGGGAA4040.10744195076286445No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG3980.1058462782267823No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT3940.10478249653606087No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG3890.10345276942265909No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT3880.10318682399997872No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT3860.10265493315461802No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG3860.10265493315461802No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC3840.10212304230925728No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC3830.10185709688657694No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAAAAGCAATGCTATGTACTGGGTCCGCCAGGCTCCAGGCAAGGGGCT3800.10105926061853587No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)