FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004718040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004718040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376017
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACAGTGTGTTCTTGGAATTGTCTCTGG12900.34306959525766123No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12590.33482528715457016No Hit
GGGTATAACTAGGTGGTTAGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTGAGTTACTGATCTATGGTGCATC10590.28163620261849864No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT8590.2284471180824271No Hit
CTCTAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTACATAGCATTGCTTTTGAAGGTGAATCCAGAGGCTGCAC7850.20876715680408067No Hit
GTGTATTACTGTGCGAGAGACCGAGTGGTGATAACTTCCAACTGGATCGACCCCTGGGGCCAGGGAACCCTGGTC7590.20185257581439137No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA7520.19999095785562887No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7440.197863394474186No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7350.1954698856700628No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6890.18323639622676635No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6850.1821726145360449No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC6820.18137477826800386No Hit
GATCTATGGTGCATCCAGTTTGCAAAGCGGGGTCCCATCAAGGTTCAGCGGCAGTGGGTCTGGGACAGATTTC6490.17259857931955203No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATAGTGAGAGTGAAATCTGTC6270.16674778002058419No Hit
GGCTAACAGTTTCCCTATCACTTTCGGCCCTGGGACCAAAGTGGATGTCAAACGAACTGTGGCTGCACCAT6120.1627585986803788No Hit
GCTATGTACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTAGAGTGGGTGGCATTTATATCATATGATGGAAGGAAT6090.16196076241233776No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT5660.15052510923708237No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG5550.14759970958759844No Hit
GGGCTAGAGTGGGTGGCATTTATATCATATGATGGAAGGAATAAAAACTACGCAGACTCCGTGAAGGGCCGATTC5470.14547214620615556No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG5460.1452062007834752No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5410.14387647367007342No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5340.14201485571131092No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC5270.14015323775254843No Hit
CACTATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTTCCCTATCACTTT5170.13749378352574484No Hit
GTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCCCAGGGCCGAAAGTGATAGGGAAACTGTTAGCCTGTTGAC5130.13643000183502343No Hit
GTTATACCCTGACTCGCCCGACAAGTGATGGTGACTGTGTCTCCTACAGA5000.13297271134017877No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATA5000.13297271134017877No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG4980.13244082049481806No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC4960.13190892964945733No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA4750.1263240757731698No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC4630.12313273070100553No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG4630.12313273070100553No Hit
GTATTACTGTGCGAGAGACCGAGTGGTGATAACTTCCAACTGGATCGACCCCTGGGGCCAGGGAACCCTGGTCAC4620.12286678527832519No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG4570.12153705816492338No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT4520.12020733105152161No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG4490.11940949478348054No Hit
GTACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTAGAGTGGGTGGCATTTATATCATATGATGGAAGGAATAAAA4480.11914354936080017No Hit
GTCTGGGACAGATTTCACTCTCACTATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGG4440.11807976767007876No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG4430.11781382224739839No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTG4400.11701598597935732No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4380.11648409513399662No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCC4330.11515436802059481No Hit
GTCCCAGACCCACTGCCGCTGAACCTTGATGGGACCCCGCTTTGCAAACTGGATGCACCATAGATCAGTAACTC4290.1140905863298734No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC4260.11329275006183231No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC4210.11196302294843052No Hit
CCATAGATCAGTAACTCAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGC4200.11169707752575017No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT4080.10850573245358588No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC4010.10664411449482337No Hit
GTGTATTACTGTGCGAGAGACCGAGTGGTGATAACTTCCAACTGGATCGACCCCTGGGGCCAGGGAACCCTGGT3940.10478249653606087No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCCCAGGGCCGAAAGTGATAGGGAA3920.10425060569070016No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG3910.1039846602680198No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG3900.10371871484533943No Hit
GAATAAAAACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACACTGTAT3900.10371871484533943No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG3850.10238898773193765No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC3820.10159115146389658No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC3800.10105926061853587No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG3780.10052736977317514No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG3780.10052736977317514No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC3780.10052736977317514No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)